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首页> 外文期刊>Biomaterials >Synthesis of photoreactive pullulan for surface modification.
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Synthesis of photoreactive pullulan for surface modification.

机译：用于表面改性的光反应性支链淀粉的合成。

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Photoreactive pullulan was prepared, the polymer was photoimmobilized on polymeric or organic surfaces, and its interactions with a protein and a cell type were investigated. The photoreactive pullulan was synthesized by a coupling reaction with 4-azidobenzonic acid. Surface modification was carried out in the presence or absence of a micropatterned photomask containing 100mum transparent stripes with 150mum gaps, making it easy to confirm the immobilization. By the micropatterning method, immobilization of the photoreactive pullulan on polystyrene, polyethylene, and silane-coupled glass was confirmed. Contact angles were measured on the unpatterned surfaces. Although the original surfaces have different contact angles, the contact angle on Az-pullulan-immobilized surface was the same on all surfaces. This result demonstrated that photoimmobilization completely covered the surface with Az-pullulan. Protein adsorption was investigated using fluorescently labeled albumin applied to the micropatterned surface: fluorescence microscopy demonstrated that adsorption was reduced on the pullulan-immobilized regions. Culture of RAW264 cells, derived from mouse leukemic monocytes, on the micropatterned surface for 22h showed that cells did not adhere to the immobilized pullulan regions. In conclusion, photoreactive pullulan was covalently immobilized on various surfaces and tended to reduce interactions with proteins and cells.
机译：制备光反应性支链淀粉，将聚合物光固定在聚合物或有机表面上，并研究其与蛋白质和细胞类型的相互作用。通过与4-叠氮基苯甲酸的偶联反应合成光反应性支链淀粉。在有或没有微图案光掩模的情况下进行表面修饰，该微图案光掩模包含具有150μm间隙的100μm透明条纹，因此易于确认固定。通过微图案化方法，确认了光反应性支链淀粉固定在聚苯乙烯，聚乙烯和硅烷偶联玻璃上。在无图案的表面上测量接触角。尽管原始表面具有不同的接触角，但在固定有Az-普鲁兰胶的表面上的接触角在所有表面上均相同。该结果表明光固定化完全被Az-普鲁兰聚糖覆盖。使用荧光标记的白蛋白应用于微图案化表面，研究了蛋白质的吸附：荧光显微镜显示吸附在支链淀粉固定的区域上减少了。在微图案表面上培养源自小鼠白血病单核细胞的RAW264细胞22h，表明细胞不粘附于固定的支链淀粉区域。总之，光反应性支链淀粉被共价固定在各种表面上，并倾向于减少与蛋白质和细胞的相互作用。

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