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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Vibrational spectroscopic studies of solid recombinant bovine growth hormone and related growth hormone analogs
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Vibrational spectroscopic studies of solid recombinant bovine growth hormone and related growth hormone analogs

机译：固体重组牛生长激素及相关生长激素类似物的振动光谱研究

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摘要

Infrared and Raman spectra have been obtained for lyophilized recombinant bovine growth hormone (r-bGH), partially reduced, and completely reduced r-bGH, plus a tryptic digest fragment of r-bGH. Amide I and II data indicate r-bGH to have substantial helical character. Partially reduced r-bGH, in which the carboxyl terminal disulfide bridge (residues 181, 189) has been cleaved, has slightly less helical content than r-bGH. The spectral data indicate that breaking the carboxyl terminal cystine link produces only localized structural alterations. The additional cleavage of the second disulfide bridge (residues 53 164) leads to a further decrease in helix content, accompanied by increases in #beta#-sheet and disordered structures. A tryptic digest r-bGH fragment (residues 96-133), which contains a small amount of biological activity (approx=10 percent), has predominantly helical structure.
机译：已获得冻干重组牛生长激素（r-bGH）的红外光谱和拉曼光谱，部分还原和完全还原了r-bGH，以及胰蛋白酶消化的r-bGH片段。酰胺I和II数据表明r-bGH具有明显的螺旋特征。部分还原的r-bGH的螺旋含量略低于r-bGH，其中羧基末端的二硫键（残基181、189）已被裂解。光谱数据表明，破坏羧基末端胱氨酸连接仅产生局部结构改变。第二二硫键（残基53 164）的额外裂解导致螺旋含量进一步降低，伴随着＃beta＃-折叠和无序结构的增加。含有少量生物活性（约10％）的胰蛋白酶消化r-bGH片段（残基96-133）主要具有螺旋结构。

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