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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Photoinduced oxygen dynamics in lyophilized hemoglobin
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Photoinduced oxygen dynamics in lyophilized hemoglobin

机译：冻干血红蛋白中的光诱导氧动力学

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Reversible laser induced deoxygenation in the lyophilized phase of hemoglobin is demonstrated by means of resonant Raman scattering, luminescence, and optical transmission. Specific Raman modes, which are both sensitive to the spin states of Fe(II) in the hemes and resonant in the visible, are monitored as a function of time to evaluate the effect of the illuminating laser. These modes act as in-situ markers of the oxygen content of the protein. The reversible photoinduced deoxygenation can be observed through both the Raman spin-markers and the optical transmission experiments. In the former, reversible changes in the intensities of specific Raman modes are observed, while in the latter, the oscillator strength of the two main absorptions of oxyhemoglobin in the visible are seen to vary accordingly. The luminescence in lyophilized hemoglobin is found to have at least two different contributions, (i) a resonant component with the Raman modes and; (ii) a nonresonant contribution, which increases at high input laser powers and eventually masks the Raman signals. The nonresonant contribution is the luminescence of the photoproduct achieved by thermal denaturation of the protein and remains standing as a permanent nonreversible damage in the illuminated spot. Semiempirical electronic calculations of the wavefunction and total energy of the iron porphyrin reveal the underlying physical origin of the laser induced deoxygenation process in the hemes and are also presented. (C) 2000 Elsevier Science B.V. All rights reserved. [References: 51]
机译：借助于共振拉曼散射，发光和光学传输证明了血红蛋白冻干阶段中可逆的激光诱导的脱氧。监视特定的拉曼模式，这些模式既对血红素中的Fe（II）的自旋态敏感，又在可见光中发生共振，它们是随时间变化的函数，以评估照明激光的效果。这些模式充当蛋白质中氧含量的原位标记。可逆的光诱导脱氧可以通过拉曼自旋标记和光学透射实验来观察。在前者中，观察到特定拉曼模式强度的可逆变化，而在后者中，可见的氧合血红蛋白的两个主要吸收的振子强度相应地变化。发现冻干的血红蛋白中的发光至少具有两种不同的作用：（i）具有拉曼模式的共振成分;和（ii）非共振贡献，在高输入激光功率下会增加，并最终掩盖拉曼信号。非共振的贡献是通过蛋白质的热变性实现的光产物的发光，并且在照明的光斑中作为永久的不可逆损伤而保持不变。卟啉铁的波函数和总能量的半经验电子计算揭示了血红素中激光诱导的脱氧过程的潜在物理起源，并进行了介绍。 （C）2000 Elsevier Science B.V.保留所有权利。 [参考：51]

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