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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Preparation of Z-L-Phe-OH-NBD imprinted microchannel and its molecular recognition study
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Preparation of Z-L-Phe-OH-NBD imprinted microchannel and its molecular recognition study

机译：Z-L-Phe-OH-NBD印迹微通道的制备及其分子识别研究

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An integrated microchip was presented for selective recognition of Z-(L)-Phe-OH-NBD, using molecular imprinting technique. Molecularly imprinted polymer (MIP) were prepared by copolymerization in the presence of template molecule Z-(L)-Phe-OH-NBD, in which methacrylic acid and 4-vinylpyridine were used as functional monomers and ethylene dimethacrylate used as crosslinker. Imprinted polymer particles were introduced into a microchannel fabricated with a new material i.e. poly(methylvinylsiioxane) by simply rapid prototyping method. Imprinted effects were evaluated by laser-induced fluorescence (LIF) detection where the results indicated that good selective recognition for Z-(L)-PheOH-NBD in the imprinted polymer was obtained; the adsorption percentage of Z-L-F-Phe-OH-NBD was 61%. In contrast to conventional molecular imprinting analysis. integration shortened overall analysis time from 4 h to 10 min. (c) 2004 Elsevier B.V. All rights reserved.
机译：提出了一种集成的微芯片，用于使用分子印迹技术选择性识别Z-（L）-Phe-OH-NBD。在模板分子Z-（L）-Phe-OH-NBD存在下，通过共聚反应制备分子印迹聚合物（MIP），其中甲基丙烯酸和4-乙烯基吡啶为功能单体，乙撑二甲基丙烯酸酯为交联剂。通过简单的快速原型制作方法，将印迹的聚合物颗粒引入由新材料即聚（甲基乙烯基硅氧烷）制成的微通道中。通过激光诱导荧光（LIF）检测评估印迹效果，结果表明，该印迹聚合物对Z-（L）-PheOH-NBD具有良好的选择性识别。 Z-L-F-Phe-OH-NBD的吸附率为61％。与常规分子印迹分析相反。集成将整体分析时间从4小时缩短到10分钟。 （c）2004 Elsevier B.V.保留所有权利。

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