Study on the binding of cerium(III) ion and spermine to TRNA~(Phe)

Jian Xin Meng; Hua Ming Tu; Meng Lian Gong; Yan Sheng Yang; Jian Ying Zhou; Hong lie Zhang

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Study on the binding of cerium(III) ion and spermine to TRNA~(Phe)

机译：铈（III）离子和精胺与TRNA〜（Phe）结合的研究

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Fluorescence of Ce(III) aqua ion at pH 6.0 is found to be in good linear relationship with its concentration, and the intensity is strong enough to be employed to determine its concentration. TRNA~(Phe) evidently quench this fluorescence. Fluorescencetitration experiments were performed to Ce(III)-tRNA~(Phe) system, the binding number and association constant was estimated with a Scatchard plot. Two classes of binding sites with association constant of 5.2 x 10~7 and 4.4 x 10~6 M, respectively, werefound. Addition of spermine slightly decrease binding number and association constant of Ce(III) ion.

机译：发现在pH 6.0的Ce（III）qua离子的荧光与其浓度具有良好的线性关系，并且强度足够强，可以用来确定其浓度。 TRNA〜（Phe）明显淬灭了这种荧光。对Ce（III）-tRNA〜（Phe）体系进行了荧光滴定实验，结合Scatchard图估计了结合数和缔合常数。发现了两类结合位点，其结合常数分别为5.2 x 10〜7和4.4 x 10〜6M。添加精胺会略微降低Ce（III）离子的结合数和缔合常数。

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《Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy》 |1997年第10期|共4页
作者
Jian Xin Meng; Hua Ming Tu; Meng Lian Gong; Yan Sheng Yang; Jian Ying Zhou; Hong lie Zhang;
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正文语种 eng
中图分类分析化学;
关键词
Cerium(III) ion; Fluorescence; Spermine;

机译：铈离子荧光精胺;

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专利

1. Study on the binding of cerium(III) ion and spermine to TRNA~(Phe) [J] . Jian Xin Meng, Hua Ming Tu, Meng Lian Gong, Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy . 1997,第10期

机译：铈（III）离子和精胺与TRNA〜（Phe）结合的研究
2. Regioselective binding of spermine, N1,N12-bismethylspermine, and N1,N12-bisethylspermine to tRNA Phe as revealed by 750 MHz 1H-NMR and its possible correlation with cell cycling and cytotoxicity [J] . Aldonia Valasinas, Benjamin Frydman, Carl W. Porter, Journal of the Brazilian Chemical Society . 1999,第4期

机译：750 MHz 1H-NMR揭示了精胺，N1，N12-双甲基精胺和N1，N12-双乙基精胺与tRNA Phe的区域选择性结合及其与细胞周期和细胞毒性的可能关系
3. Spermine Binds in Solution to the TψC Loop of tRNA~(Phe): Evidence from a 750 MHz ~1H-NMR Analysis [J] . Benjamin Frydman, William M. Westler, Keijiro Samejima The Journal of Organic Chemistry . 1996,第8期

机译：精胺结合到tRNA〜（Phe）TψC环的溶液中：750 MHz〜1H-NMR分析的证据
4. Protein-Oligonucleotide Complexes Characterization using Noncovalent Mass Spectrometry: Study of the tRNA binding properties of tRNA m1A58 methyltransferase. [C] . Cedric ATMANENE, Pierre BARRAUD, Frederic DARDEL, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：蛋白质 - 寡核苷酸复合物的表征使用非共价质谱：TRNA M1A58甲基转移酶的TRNA结合性能研究。
5. Structure-based design of mutant proteins: I. Molecular docking studies of amino acid binding to wild-type aminoacyl-tRNA synthetases. II. Structure-based design of mutant aminoacyl-tRNA synthetases for non-natural amino acid incorporation. [D] . Zhang, Deqiang. 2003

机译：基于结构的突变蛋白设计：I.氨基酸与野生型氨酰基tRNA合成酶结合的分子对接研究。二。非天然氨基酸掺入的突变型氨酰基-tRNA合成酶的基于结构的设计。
6. New photoactivatable structural and affinity probes of RNAs: specific features and applications for mapping of spermine binding sites in yeast tRNA(Asp) and interaction of this tRNA with yeast aspartyl-tRNA synthetase. [O] . A Garcia, R Giegé, J P Behr 1990

机译：RNA的新的光激活结构和亲和力探针：酵母tRNA（Asp）中精胺结合位点的定位及其与酵母天冬氨酰-tRNA合成酶相互作用的特定功能和应用。
7. New photoactivatable structural and affinity probes of RNAs: specific features and applications for mapping of spermine binding sites in yeast tRNA(Asp) and interaction of this tRNA with yeast aspartyl-tRNA synthetase. [O] . Garcia, A, Giegé, R, Behr, J P 1990

机译：RNA的新的光激活结构和亲和力探针：酵母tRNA（Asp）中精胺结合位点的定位及其与酵母天冬氨酰-tRNA合成酶相互作用的特定功能和应用。

Study on the binding of cerium(III) ion and spermine to TRNA~(Phe)

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