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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Characterization of parallel and antiparallel G-tetraplex structures by vibrational spectroscopy
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Characterization of parallel and antiparallel G-tetraplex structures by vibrational spectroscopy

机译：通过振动光谱表征平行和反平行G-四链体结构

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摘要

A series of G-rich oligonucleotides able to form tetraplexes has been studied by FTIR spectroscopy. Characteristic markers of the formation of guanine tetrads are given. Moreover, we propose a new marker discriminating between parallel and antiparallel tetraplexes: the position of the C6=O6 guanine carbonyl stretching vibration. In intermolecular parallel tetrameric structures formed by four separate strands this absorption is observed at 1693 cm(-1) while for antiparallel tetrameric structures, either intramolecular or formed by dimerization of hairpins, this vibrational mode is observed at 1682 cm(-1). These shifts to higher wavenumbers, when compared to the position of a free guanine C6=O6 carbonyl stretching vibration observed at 1666 cm(-1) (Delta v=27 cm(-1) for parallel tetraplexes and Delta v = 16 cm(-1) for antiparallel tetraplexes) reflect different strand orientations in the structures. This marker has been used to evidence the possibility of an antiparallel-parallel tetraplex reorganization for Oxytricha nova d(G(4)T(4)G(4)) and d((G(4)T(4))(3)G(4)) and human d(G(3)T(2)AG(3)) telomeric sequences induced by Na+/K+ or Na+/Ca2+ ion exchange. Formation of the guanine tetrads, characterization of the phosphate geometries and of the sugar conformations have also been obtained by FTIR for the different tetraplexes. (c) 2005 Elsevier B.V. All rights reserved.
机译：通过FTIR光谱研究了一系列能够形成四重体的富含G的寡核苷酸。给出了鸟嘌呤四联体形成的特征标记。此外，我们提出了区分平行和反平行四重体的新标记：C6 = O6鸟嘌呤羰基拉伸振动的位置。在由四个分开的链形成的分子间平行四聚体结构中，在1693 cm（-1）处观察到这种吸收，而对于分子内或由发夹二聚形成的反平行四聚体结构，则在1682 cm（-1）处观察到这种振动模式。与在1666 cm（-1）处观察到的游离鸟嘌呤C6 = O6羰基拉伸振动的位置相比，这些位移向更高的波数移动（对于平行四重体，Delta v = 27 cm（-1），而Delta v = 16 cm（- 1）对于反平行四链体）反映结构中不同的链取向。此标记已被用来证明Oxytricha nova d（G（4）T（4）G（4））和d（（G（4）T（4））（3）进行反平行四重体重组的可能性G（4））和人d（G（3）T（2）AG（3））端粒序列，由Na + / K +或Na + / Ca2 +离子交换诱导。鸟嘌呤四联体的形成，磷酸盐几何形状的特征和糖构象的表征也已通过FTIR获得了不同的四链体。 （c）2005 Elsevier B.V.保留所有权利。

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