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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Study on the influence of potassium iodate on the metabolism of Escherichia coli by intrinsic fluorescence
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Study on the influence of potassium iodate on the metabolism of Escherichia coli by intrinsic fluorescence

机译：固有荧光研究碘酸钾对大肠杆菌代谢的影响

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Intrinsic fluorescence, in particular, has the advantage over the extrinsic fluorescence of an unperturbed environment during investigation, especially in complex systems such as biological cells and tissues. Potassium iodate may restrain bacteria growth as well as it acts as an additive in the salt. The influence of potassium iodate (KIO3) on the metabolism of Escherichia coli (E. coli) is investigated for the first time with the intrinsic fluorescence of tryptophan (Trp) and reduced nicotinamide adenine dinucleotide (NADH). We found that potassium iodate may restrain the growth of E. coli as a bacteriostatic agent. When the potassium iodate concentration was below 1.32 mmol/L, the intensity of tryptophan fluorescence decreased linearly whereas the NADH fluorescence did not change. When the KIO3 concentration was over 1.32 mmol/L, the fluorescence of tryptophan and NADH increased a little and their fluorescence intensity decreased when KIO3 was over 6.67 mmol/L. And the bacteria could not continue growing if the KIO3 was over 6.67 mmol/L. We could conclude that potassium iodate has great inhibiting effects on the growth of E. coli through the pathway of protein synthesis and respiratory chain. (c) 2005 Elsevier B.V. All rights reserved.
机译：特别地，固有荧光比研究期间不受干扰的环境的外部荧光具有优势，特别是在诸如生物细胞和组织的复杂系统中。碘酸钾可能会抑制细菌生长，并在盐中起添加剂的作用。首次利用色氨酸（Trp）和还原的烟酰胺腺嘌呤二核苷酸（NADH）的固有荧光研究了碘酸钾（KIO3）对大肠杆菌（E. coli）代谢的影响。我们发现，碘酸钾可能会抑制作为抑菌剂的大肠杆菌的生长。当碘酸钾浓度低于1.32 mmol / L时，色氨酸荧光强度线性下降，而NADH荧光不变。当KIO3浓度超过1.32 mmol / L时，色氨酸和NADH的荧光强度略有增加，而当KIO3浓度超过6.67 mmol / L时，其荧光强度降低。如果KIO3超过6.67 mmol / L，细菌将无法继续生长。我们可以得出结论，碘酸钾通过蛋白质合成和呼吸链途径对大肠杆菌的生长具有很大的抑制作用。 （c）2005 Elsevier B.V.保留所有权利。

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