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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Spectrofluorimetric determination of trace heparin using lomefloxacin-terbium probe
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Spectrofluorimetric determination of trace heparin using lomefloxacin-terbium probe

机译：洛美沙星-ter探针荧光光谱法测定痕量肝素

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A new spectrofluorimetric method was developed for determination of trace amount of heparin (Hep). Using lomefloxacin (LOM)-terbium ion (Tb3+) as a fluorescent probe, in the buffer solution of pH 8.70, Hep can remarkably enhance the fluorescence intensity of the LOM-Tb3+ complex at lambda = 545 nm and the enhanced fluorescence intensity of Tb3+ ion is in proportion to the concentration of Hep. Optimum conditions for the determination of Hep were also investigated. The linear range for the determination of Hep was 0.6-2.0 mu g/ml and the detection limit was 45.22 ng/ml. This method is simple, practical and relatively free of interference from coexisting substances and can be successfully applied to assess Hep in biological samples. By the Rosenthanl graphic method, the association constant of Hep with the probe is 4.56 x 10(4) Ilmol and binding numbers is 18.2. Moreover, the enhancement mechanism of the fluorescence intensity in the LOM-Tb3+ system and the LOM-Tb3+-Hep system have also been discussed. (c) 2005 Elsevier B.V. All rights reserved.
机译：开发了一种新的荧光光谱法，用于测定痕量肝素（Hep）。使用洛美沙星（LOM）-ter离子（Tb3 +）作为荧光探针，在pH 8.70的缓冲溶液中，Hep可以显着增强lamda = 545 nm时LOM-Tb3 +复合物的荧光强度，并增强Tb3 +离子的荧光强度与Hep的浓度成正比。还研究了确定Hep的最佳条件。测定Hep的线性范围是0.6-2.0μg / ml，检出限是45.22 ng / ml。该方法简单，实用并且相对不受共存物质的干扰，可以成功地用于评估生物样品中的Hep。通过Rosenthanl图解法，Hep与探针的缔合常数为4.56 x 10（4）Ilmol，结合数为18.2。此外，还讨论了LOM-Tb3 +系统和LOM-Tb3 + -Hep系统中荧光强度增强的机理。 （c）2005 Elsevier B.V.保留所有权利。

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