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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Spectrofluorimetric determination of trace aluminum in diluted hemodialysis solutions
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Spectrofluorimetric determination of trace aluminum in diluted hemodialysis solutions

机译：荧光光谱法测定稀释的血液透析溶液中的痕量铝

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In this study, a spectrofluorimetric method has been developed for the determination of aluminum based on the formation of an aluminum complex with N,N'-disalicylidene-1,3-diamino-2-hydroxypropane (DSAHP). The most suitable pH, solvent medium, complex formation time, Schiff base concentration and temperature were determined. The excitation and emission wavelengths were 270 and 437 nm, respectively, in which the DSAHP-Al complex gave the maximum flurescence intensity at pH 3.0 and 6.0 in 50% dioxan-50% water medium. Under these conditions, calibration curves were obtained in three different linear limits, and was found that aluminum could be detected within the concentration limit of 0-10.0 muM and the lowest detection limit being 0.27 ng ml(-1). The stochiometry of the DSAHP-Al complex was also determined spectrofluorimetrically under optimal conditions and the molar ratio of DSAHP-Al was calculated as 2 :1. Using the developed method, aluminum was detected in hemodialysis solutions, and the results obtained were similar and comparable with those obtained using the method described in the British Pharmacopoeia within 95% confidence limits. This method can be used successfully for the routine determination of aluminum because it is quick, requires less amount of reactives, is sensitive, reliable and reproducible. (C) 2004 Elsevier B.V. All rights reserved.
机译：在这项研究中，基于与N，N'-二水杨基-1,3-二氨基-2-羟基丙烷（DSAHP）形成铝络合物的研究，开发了一种荧光光谱法测定铝。确定了最合适的pH，溶剂介质，络合物形成时间，席夫碱浓度和温度。激发和发射波长分别为270和437 nm，其中DSAHP-Al络合物在50％二恶烷50％水介质中在pH 3.0和6.0时具有最大荧光强度。在这些条件下，在三个不同的线性极限下获得了校准曲线，发现可以在0-10.0μM的浓度极限内检测到铝，最低检测极限为0.27 ng ml（-1）。还在最佳条件下通过分光荧光法测定了DSAHP-Al配合物的化学计量，并且DSAHP-Al的摩尔比被计算为2∶1。使用开发的方法，可以在血液透析溶液中检测到铝，所获得的结果与使用英国药典中描述的方法所获得的结果相似，并且在95％的置信限内。该方法快速，所需的反应物数量少，灵敏，可靠且可重现，因此可以成功地用于铝的常规测定。 （C）2004 Elsevier B.V.保留所有权利。

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