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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Quantitative photoluminescence of broad band absorbing melanins: a procedure to correct for inner filter and re-absorption effects
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Quantitative photoluminescence of broad band absorbing melanins: a procedure to correct for inner filter and re-absorption effects

机译:宽带吸收性黑色素的定量光致发光:校正内部过滤器和重吸收效果的程序

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摘要

We report methods for correcting the photoluminescence emission and excitation spectra of highly absorbing samples for re-absorption and inner filter effects. We derive the general form of the correction, and investigate various methods for determining the parameters. Additionally, the correction methods are tested with highly absorbing fluorescein and melanin (broadband absorption) solutions; the expected linear relationships between absorption and emission are recovered upon application of the correction, indicating that the methods are valid. These procedures allow accurate quantitative analysis of the emission of low quantum yield samples (such as melanin) at concentrations where absorption is significant. (c) 2004 Elsevier B.V. All rights reserved.
机译:我们报告纠正高吸收性样品的光致发光发射和激发光谱的方法,以实现再吸收和内部滤光片效果。我们得出了校正的一般形式,并研究了确定参数的各种方法。此外,校正方法还需使用高吸收性荧光素和黑色素(宽带吸收)溶液进行测试。应用校正后,吸收和发射之间的预期线性关系得以恢复,表明该方法是有效的。这些程序可以对吸收显着的浓度下的低量子产率样品(例如黑色素)的发射进行准确的定量分析。 (c)2004 Elsevier B.V.保留所有权利。

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