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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >The antioxidative power AP - A new quantitative time dependent (2D) parameter for the determination of the antioxidant capacity and reactivity of different plants
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The antioxidative power AP - A new quantitative time dependent (2D) parameter for the determination of the antioxidant capacity and reactivity of different plants

机译:抗氧化能力AP-一种新的定量时间依赖性(2D)参数,用于确定不同植物的抗氧化能力和反应性

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In the last decade, naturally occurring antioxidants continue to play an important role in the food-supplement industry. The content of antioxidants in a plant depends on the species, temperature, humidity, period of growth, harvest month, part of the plant used and many other variables. Herein, we present a new method able to determine the all over antioxidative power (AP) of plant extracts or lyophilised plant parts based on the reducing activity against a stable test radical. The method is performed by ESR spectroscopy and is based on the well-known 1,1-diphenyl-2-picryl-hydrazil (DPPH) method with the major difference that both the antioxidative capacity and the antioxidative activity are used to characterise an antioxidant. The resulting antioxidative power is expressed in antioxidative units (AU), where 1 AU corresponds to the activity of a 1 ppm solution of Vitamin C as a benchmark. This method allows a rapid, unexpensive and general applicable technique for the measurement of the antioxidative power of very different kinds of substances. The inclusion of the kinetic behaviour of the reducing process of the antioxiclant for the determination of the AP allows the identification of the main antioxidant present in a sample. Herein, we present the application example of seeds, sprouts and adult parts of dandelion, amaranth, quinoa, fenugreek, broccoli, red clover and mugwort, where the AP method permits to characterise the plants with the highest antioxidant capacity and reaction velocity. The method permits to select active plant extracts for the food and nutrition industry. (c) 2005 Elsevier B.V. All rights reserved.
机译:在过去的十年中,天然存在的抗氧化剂继续在食品补充行业中发挥重要作用。植物中抗氧化剂的含量取决于物种,温度,湿度,生长时期,收获月份,所用植物的一部分以及许多其他变量。本文中,我们提出了一种新方法,该方法基于对稳定测试基团的还原活性,可确定植物提取物或冻干植物部位的全部抗氧化能力(AP)。该方法通过ESR光谱法进行,并且基于众所周知的1,1-二苯基-2-吡啶-肼(DPPH)方法,主要区别在于抗氧化能力和抗氧化活性均用于表征抗氧化剂。产生的抗氧化能力以抗氧化单位(AU)表示,其中1 AU对应于1 ppm维生素C溶液作为基准的活性。这种方法提供了一种快速,廉价且通用的技术来测量非常不同种类的物质的抗氧化能力。包含用于确定AP的抗氧剂还原过程的动力学行为,可以鉴定样品中存在的主要抗氧化剂。在此,我们介绍蒲公英,a菜,藜麦,胡芦巴,西兰花,红三叶草和艾蒿的种子,芽和成年部分的应用实例,其中AP方法可以表征具有最高抗氧化能力和反应速度的植物。该方法允许选择用于食品和营养行业的活性植物提取物。 (c)2005 Elsevier B.V.保留所有权利。

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