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首页> 外文期刊>Structure >A structural code for discriminatina between mum transcription signals revealed by the feast/famine regulatory protein DM1 in complex with ligands
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A structural code for discriminatina between mum transcription signals revealed by the feast/famine regulatory protein DM1 in complex with ligands

机译：餐/饥荒调节蛋白DM1与配体复合时揭示的妈妈转录信号之间区别的结构代码

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摘要

Feast/famine regulatory proteins (FFRPs) comprise the largest group of archaeal transcription factors. Crystal structures of an FFRP, DM1 from Pyrococcus, were determined in complex with isoleucine, which increases the association state of DM1 to form octamers, and with selenomethionine, which decreases it to maintain dimers under some conditions. Asp39 and Thr/Ser at 69-71 were identified as being important for interaction with the ligand main chain. By analyzing residues surrounding the ligand side chain, partner ligands were identified for various FFRPs from Pyrococcus, e.g., lysine facilitates homo-octamerization of FL11, and arginine facilitates hetero-octamerization of FL11 and DM1. Transcription of the fill gene and lysine synthesis are regulated by shifting the equilibrium between association states of FL11 and by shifting the equilibrium toward association with DM1, in response to amino acid availability. With FFRPs also appearing in eubacteria, the origin of such regulation can be traced back to the common ancestor of all extant organisms, serving as a prototype of transcription regulations, now highly diverged.
机译：盛宴/饥荒调节蛋白（FFRP）构成最大的古细菌转录因子。 FFRP的晶体结构，来自火球菌的DM1，是与异亮氨酸复合物测定的，异亮氨酸可增加DM1的缔合状态以形成八聚体，而硒代蛋氨酸可降低其在某些条件下的二聚体。鉴定出在69-71的Asp39和Thr / Ser对于与配体主链的相互作用很重要。通过分析配体侧链周围的残基，鉴定了来自火球菌的各种FFRP的配体配体，例如，赖氨酸促进FL11的均八聚化，而精氨酸促进FL11和DM1的异八聚化。通过改变FL11的缔合状态之间的平衡并且通过使平衡向与DM1缔合的方向移动来响应氨基酸的可用性来调节填充基因的转录和赖氨酸的合成。由于FFRPs也出现在真细菌中，所以这种调节的起源可以追溯到所有现存生物的共同祖先，它们是转录调节的原型，现已高度分化。

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