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首页> 外文期刊>Structure >Portrayal of Complex Dynamic Properties of Sugarcane Defensin 5 by NMR: Multiple Motions Associated with Membrane Interaction
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Portrayal of Complex Dynamic Properties of Sugarcane Defensin 5 by NMR: Multiple Motions Associated with Membrane Interaction

机译：核磁共振描绘甘蔗防御素5复杂动态特性：与膜相互作用相关的多重运动。

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摘要

Defensins are essentially ancient natural antibiotics with potent activity extending from lower organisms to humans. Sd5 is a recently described antifungal defensin that appears to be the result of a recent gain of function. We reported here the solution NMR structure of Sd5 and characterized the backbone dynamics in the free state and in the presence of membrane models. 15N relaxation dispersion measurements indicate intrinsic conformational exchange processes, showing two clear distinct kex, 490 and 1800 s-1. These multiple motions may be related to transient twisting or breathing of the a helix and b sheet. The stages of membrane recognition and disruption by Sd5 over a large timescale range were mapped and demonstrated that Sd5 in solution sampled an ensemble of different conformations, of which a subset is selected upon membrane binding. Defensins share similar structures, but we demonstrated here that their dynamics can be extremely diverse.
机译：防御素本质上是古老的天然抗生素，其有效活性从低等生物扩展到人类。 Sd5是最近描述的抗真菌防御素，似乎是近期功能增强的结果。我们在这里报告了Sd5的溶液NMR结构，并表征了在自由状态和存在膜模型时的骨架动力学。 15N弛豫分散度测量表明内在构象交换过程，显示出两个明显不同的Kex，即490和1800 s-1。这些多个动作可能与a螺旋和b片的瞬时扭曲或呼吸有关。绘制了在较大时间范围内通过Sd5进行膜识别和破坏的阶段图，并证明溶液中的Sd5采样了一组不同构象的集合，其中一个子集是在膜结合后选择的。防御素具有相似的结构，但我们在此处证明了它们的动力学可能极为不同。

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