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首页> 外文期刊>Surgical Endoscopy >Does thermodynamic stability of peritoneal collagen change during laparoscopic cholecystectomies? A differential scanning calorimetry (DSC) study
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Does thermodynamic stability of peritoneal collagen change during laparoscopic cholecystectomies? A differential scanning calorimetry (DSC) study

机译：腹腔镜胆囊切除术期间腹膜胶原蛋白的热力学稳定性会改变吗？差示扫描量热法（DSC）研究

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Background: Carbon dioxide pneumoperitoneum used during laparoscopic surgeries alters the integrity of the peritoneum and results in denudation of the basal lamina that might cause altered immune response, inhibited fibrinolysis, hypoxia, and acidosis. The changes in the structure of pneumoperitoneum were described as bulging of mesothelial cells, irregular cell junction's cell membrane degradation, and mesodermal edema. As denaturation of peritoneal proteins reflects overall condition of its structure and interactions with the surrounding molecules, the physical status of collagen was assessed on the basis of parameters of thermal denaturation measured by DSC method. Methods: Twenty-four female patients operated on due to cholelithiasis were enrolled in this study. Laparoscopic cholecystectomy was performed using standard four-trocar technique, and standard values of insufflated carbon dioxide pneumoperitoneum were used. After trocar placement, the first collection of peritoneal sample (sample A) was performed. The second peritoneal sample (sample B) was collected after the removal of gall bladder. Differential scanning calorimetry (Q200 calorimeter, TA Instruments) was performed on samples defrosted at room temperature. Results: In all samples of peritoneum, a nonreversible endothermal process recognized as denaturation was observed. Sample B obtained at the end of surgery did not differ from sample A obtained at the beginning in terms of all parameters under study. Temperature of denaturation in A and B was correlated only marginally, but enthalpy and specific heat were significantly correlated. The analysis of data from DSC measurements did not reveal differences in physical stability of collagen in peritoneal samples obtained at the beginning and at the end of surgery. Significant negative correlations between duration of CO2 pneumoperitoneum and enthalpy of denaturation in sample B were found. Conclusions: Differences in enthalpy of denaturation may reflect a quantitative relation between amount of native collagen molecules in the sample and other, non-collagenous components or impaired collagen.
机译：背景：腹腔镜手术中使用的二氧化碳气腹会改变腹膜的完整性，并导致基底层的剥落，这可能导致免疫反应改变，抑制纤维蛋白溶解，缺氧和酸中毒。气腹的结构变化被描述为间皮细胞膨出，不规则的细胞交界处的细胞膜降解和中胚层水肿。由于腹膜蛋白的变性反映了其结构的整体状况以及与周围分子的相互作用，因此，基于通过DSC方法测量的热变性的参数来评估胶原的物理状态。方法：本研究纳入了24例因胆石症而接受手术的女性患者。腹腔镜胆囊切除术采用标准的四套管针技术进行，并使用吹入二氧化碳气腹的标准值。放置套管针后，进行腹膜样品（样品A）的第一次收集。在除去胆囊后收集第二个腹膜样品（样品B）。对在室温下除霜的样品进行差示扫描量热法（Q200热量计，TA Instruments）。结果：在所有腹膜样品中，均观察到不可逆的吸热过程，即变性。就所研究的所有参数而言，在手术结束时获得的样品B与开始时获得的样品A没有区别。 A和B中的变性温度仅略有相关，而焓和比热则显着相关。 DSC测量数据的分析未揭示在手术开始和结束时获得的腹膜样品中胶原蛋白的物理稳定性差异。发现样品B中的CO2气腹持续时间与变性焓之间存在显着的负相关。结论：变性焓的差异可能反映了样品中天然胶原蛋白分子的数量与其他非胶原蛋白成分或受损的胶原蛋白之间的定量关系。

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