...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Surgical Endoscopy >Laparoscopic surgery and the parasympathetic nervous system.
【24h】

Laparoscopic surgery and the parasympathetic nervous system.

机译：腹腔镜手术和副交感神经系统。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Laparoscopic surgery preserves the immune system and has anti-inflammatory properties. CO2 pneumoperitoneum attenuates lipopolysaccharide (LPS)-induced cytokine production and increases survival. We tested the hypothesis that CO2 pneumoperitoneum mediates its immunomodulatory properties via stimulation of the cholinergic pathway. METHODS: In the first experiment, rats (n = 68) received atropine 1 mg/kg or saline injection 10 min prior to LPS injection and were randomization into four 30-min treatment subgroups: LPS only control, anesthesia control, CO2 pneumoperitoneum, and helium pneumoperitoneum. In a second experiment, rats (n = 40) received atropine 2 mg/kg or saline 10 min prior to randomization into the same four subgroups described previously. In a third experiment, rats (n = 96) received atropine 2 mg/kg or saline 10 min prior to randomization into eight 30-min treatment subgroups followed by LPS injection: LPS only control; anesthesia control; and CO2 or helium pneumoperitoneum at 4, 8, and 12 mmHg. In a fourth experiment, rats (n = 58) were subjected to bilateral subdiaphragmatic truncal vagotomy or sham operation. Two weeks postoperatively, animals were randomized into four 30-min treatment subgroups followed by LPS injection: LPS only control, anesthesia control, CO2 pneumoperitoneum, and helium pneumoperitoneum. Blood samples were collected from all animals 1.5 h after LPS injection, and cytokine levels were determined by enzyme-linked immunosorbent assay. RESULTS: Serum tumor necrosis factor-alpha (TNF-alpha) levels were consistently suppressed among the saline-CO2 pneumoperitoneum groups compared to saline-LPS only control groups (p < 0.05 for all four experiments). All chemically vagotomized animals had significantly reduced TNF-alpha levels compared to their saline-treated counterparts (p < 0.05 for all), except among the CO2 pneumoperitoneum-treated animals. Increasing insufflation pressure with helium eliminated differences (p < 0.05) in TNF-alpha production between saline- and atropine-treated groups but had no effect among CO2 pneumoperitoneum-treated animals. Finally, vagotomy (whether chemical or surgical) independently decreased LPS-stimulated TNF-alpha production in all four experiments. CONCLUSION: CO2 pneumoperitoneum modulates the immune system independent of the vagus nerve and the cholinergic pathway.
机译：背景：腹腔镜手术可保留免疫系统并具有抗炎特性。 CO2气腹可减弱脂多糖（LPS）诱导的细胞因子产生并提高生存率。我们测试了CO2气腹通过刺激胆碱能途径介导其免疫调节特性的假说。方法：在第一个实验中，大鼠（n = 68）在LPS注射前10分钟接受阿托品1 mg / kg或盐水注射，并随机分为四个30分钟的治疗亚组：仅LPS对照，麻醉对照，CO2气腹和氦气腹膜在第二个实验中，大鼠（n = 40）在随机分为10个相同的亚组之前10分钟接受2 mg / kg阿托品或生理盐水。在第三个实验中，大鼠（n = 96）在随机分为8个30分钟的治疗亚组之前的10分钟内接受2 mg / kg的阿托品或生理盐水，然后进行LPS注射。麻醉控制以及4、8和12 mmHg的CO2或气腹氦气。在第四个实验中，对大鼠（n = 58）进行双侧dia肌下截肢迷走神经切断术或假手术。术后两周，将动物随机分为四个30分钟的治疗亚组，然后进行LPS注射：仅LPS对照，麻醉对照，CO2气腹和氦气腹。 LPS注射后1.5小时，从所有动物中收集血样，并通过酶联免疫吸附测定法测定细胞因子水平。结果：与仅盐水-LPS对照组相比，盐水-CO2气腹膜组中的血清肿瘤坏死因子-α（TNF-alpha）水平始终受到抑制（所有四个实验的p <0.05）。与经生理盐水处理的动物相比，所有经化学迷走神经处理的动物的TNF-α水平均显着降低（全部p <0.05），而经CO2气腹治疗的动物除外。用氦气增加吹入压力消除了盐水和阿托品治疗组之间TNF-α产生的差异（p <0.05），但在经CO2气腹治疗的动物中没有影响。最后，在所有四个实验中，迷走神经切开术（无论是化学疗法还是外科手术）均独立降低LPS刺激的TNF-α产生。结论：CO2气腹可独立于迷走神经和胆碱能途径调节免疫系统。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号