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首页> 外文期刊>Surgical Endoscopy >Impact of laparoscopic gases on peritoneal microenvironment and essential parameters of cell function.
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Impact of laparoscopic gases on peritoneal microenvironment and essential parameters of cell function.

机译：腹腔镜气体对腹膜微环境和细胞功能基本参数的影响。

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Several experimental studies confirm the hypotheses that laparoscopic gases influence the development of tumor metastases [12, 14, 23]. The mechanism for this alteration of malignant tumor growth is still unknown. One reason might be an influence of the in sufflation gas on essential cell function regulating parameters. To investigate the changes of the intra- and extracellular milieu, four parameters--extra- and intracellular pH, intracellular free calcium levels, and tissue oxygen partial pressure--were measured during insufflation with carbon dioxide (CO2), helium (He), or a nonhypoxic gas mixture consistent of 80% CO2 and 20% O2. STUDY DESIGN: (In vitro experiments) Intracellular calcium and pH levels were measured in DHD/K12/TRb colon adenocarcinoma cells using fluorescence imaging microscopy. (In vivo experiments) Tissue oxygen partial pressure was measured using a flexible micro catheter (Licox CMP) implanted in the abdominal wall of rats. After establishing the pneumoperitoneum an optical system and an aspirator were inserted to control the position of the micro catheter and to aspirate wound exudates for pH measurements of the wound fluid. RESULTS: Creating of pneumoperitoneum with both CO2 and helium caused a decrease in partial pressure of oxygen in the abdominal wall to about 5 mm Hg whereas insufflation with a nonhypoxic gas mixture (80% CO2 and 20% O2) induced no significant changes. The intra- and extra cellular pH values dramatically decreased during CO2 insufflation (7.4 to 6.2) in vitro. Helium caused a pH increase up to 7.6. Free intracellular calcium was enhanced during CO2 insufflation, whereas helium insufflation did not cause any changes in [Ca2+]i. Nevertheless, a significant decrease of [Ca2+]i was observed during reoxygenation following helium-induced hypoxia. CONCLUSION: Our study demonstrates that insufflation with either CO2 or He causes significant changes of intra- and extracellular parameters regulating essential cell functions such as oxidative phosphorylation to produce ATP, cell proliferation, or onset of apoptosis.
机译：几项实验研究证实了腹腔镜气体影响肿瘤转移发展的假说[12、14、23]。这种改变恶性肿瘤生长的机制仍是未知的。原因之一可能是吹入气体对基本细胞功能调节参数的影响。为了研究细胞内和细胞外环境的变化，在吹入二氧化碳（CO2），氦气（He）期间测量了四个参数-细胞外和细胞内pH，细胞内游离钙水平和组织氧分压，或浓度为80％CO2和20％O2的无氧气体混合物。研究设计：（体外实验）使用荧光成像显微镜在DHD / K12 / TRb结肠腺癌细胞中测量细胞内钙和pH值。 （体内实验）使用植入大鼠腹壁的柔性微型导管（Licox CMP）测量组织氧分压。建立气腹后，插入光学系统和抽吸器以控制微导管的位置并抽吸伤口渗出液以测量伤口液的pH。结果：同时含有CO2和氦气的气腹会导致腹壁氧气分压降低至约5 mm Hg，而采用无氧混合气体（80％CO2和20％O2）吹入则不会引起显着变化。体外CO2注入期间，细胞内和细胞外pH值均急剧降低（7.4至6.2）。氦导致pH升高至7.6。二氧化碳吹入时游离细胞内钙增加，而氦吹入并没有引起[Ca2 +] i的任何变化。然而，在氦诱导的缺氧后的复氧过程中观察到[Ca2 +] i的显着降低。结论：我们的研究表明，吹入CO2或He会引起胞内和胞外参数的显着变化，这些参数调节必需的细胞功能，例如氧化磷酸化以产生ATP，细胞增殖或凋亡开始。

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