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首页> 外文期刊>Biomaterials >Whispering gallery mode biosensor quantification of fibronectin adsorption kinetics onto alkylsilane monolayers and interpretation of resultant cellular response.
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Whispering gallery mode biosensor quantification of fibronectin adsorption kinetics onto alkylsilane monolayers and interpretation of resultant cellular response.

机译：耳语画廊模式生物传感器量化纤连蛋白在烷基硅烷单层上的吸附动力学，并解释由此产生的细胞反应。

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摘要

A Whispering Gallery Mode (WGM) biosensor was constructed to measure the adsorption of protein onto alkysilane self-assembled monolayers (SAMs) at solution concentrations unattainable with other techniques. The high sensitivity was provided by a WGM resonance excited in a silica microsphere that was functionalized with alkylsilane SAMs and integrated in a microfluidic flow cell under laminar flow conditions. It was found that FN adsorbed at biologically relevant surface densities, however, the adsorption kinetics and concentration dependent saturation values varied significantly from work published utilizing alkanethiol SAMs. Mathematical models were applied to the experimental results to interpret the observed kinetics of FN adsorption. Embryonic hippocampal neurons and skeletal myoblasts were cultured on the modified surfaces, a live--dead assay was used to determine the viability of the FN surfaces for cell culture, and major differences were noted in the biological response to the different SAMs. The high sensitivity and simplicity of the WGM biosensor, combined with its ability to quantify the adsorption of any dilute protein in a label-free assay, establishes the importance of this technology for the study of surface accretion and its effect on cellular function, which can affect biomaterials for both in vivo and in vitro applications.
机译：构建了耳语画廊模式（WGM）生物传感器，以在其他技术无法达到的溶液浓度下测量蛋白质在烷基硅烷自组装单分子膜（SAM）上的吸附。高灵敏度是由在二氧化硅微球中激发的WGM共振提供的，该WGM共振已用烷基硅烷SAMs功能化，并在层流条件下集成在微流体流通池中。发现FN吸附在生物学上相关的表面密度，但是，吸附动力学和浓度依赖性饱和值与使用烷硫醇SAMs公开的工作显着不同。将数学模型应用于实验结果以解释观察到的FN吸附动力学。在修饰的表面上培养胚胎海马神经元和骨骼肌成肌细胞，使用活死测定法确定FN表面用于细胞培养的活力，并且注意到对不同SAM的生物学反应存在主要差异。 WGM生物传感器的高灵敏度和简便性，以及在无标记测定中量化任何稀蛋白吸附的能力，确立了该技术在研究表面积聚及其对细胞功能影响方面的重要性。影响体内和体外应用的生物材料。

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