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首页> 外文期刊>Biomaterials >Protein binding mediation of biomaterial-dependent monocyte activation on a degradable polar hydrophobic ionic polyurethane
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Protein binding mediation of biomaterial-dependent monocyte activation on a degradable polar hydrophobic ionic polyurethane

机译：可降解极性疏水离子聚氨酯对生物材料依赖性单核细胞活化的蛋白质结合介导

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摘要

Protein adsorption is an important phenomenon influencing the cellular response to biomaterials. Previous studies comparing monocyte activation on a degradable polar hydrophobic ionic polyurethane (D-PHI) indicated a reduced pro-inflammatory monocyte response relative to tissue culture polystyrene (TCPS) and poly(lactide-co-glycolide) (PLGA) substrates. The present study investigated the influence of protein binding in order to gain further insight into the observed differential monocyte activation. Several proteins, identified in different relative amounts within the bound protein layers on D-PHI vs. PLGA and TCPS, were evaluated for their effect on monocyte activation. It was found that, in general, both non-coated and protein pre-adsorbed D-PHI supported a reduced pro-inflammatory response relative to PLGA, as indicated by lower levels of tumor necrosis factor-α (TNF-α) release. An initial increase in TNF-α release occurred when α 2-macroglobulin (A2M) was pre-adsorbed to D-PHI, which was shown to involve the α 2-macroglobulin receptor and was active on D-PHI but not on the two other biomaterials. This response was not observed during competitive protein binding in the presence of fetal bovine serum (FBS), suggesting that a more complex arrangement of the bound proteins and their interactions with one another, as well as with the surface chemistry of the individual biomaterials, resulted in the low-activating character of D-PHI when interacting with human monocytes.
机译：蛋白质吸附是影响细胞对生物材料反应的重要现象。先前比较可降解极性疏水离子聚氨酯（D-PHI）上单核细胞活化的研究表明，相对于组织培养聚苯乙烯（TCPS）和聚丙交酯-共-乙交酯（PLGA）底物，促炎性单核细胞反应减少。本研究调查了蛋白质结合的影响，以便获得对观察到的差异单核细胞激活的进一步了解。评估了几种蛋白质，它们在D-PHI与PLGA和TCPS的结合蛋白层中以不同的相对量被鉴定出，它们对单核细胞激活的影响。已发现，一般而言，未包被的和蛋白预吸附的D-PHI都支持相对于PLGA减少的促炎反应，如较低水平的肿瘤坏死因子-α（TNF-α）释放所表明的。当将α2-巨球蛋白（A2M）预吸附到D-PHI时，TNF-α的释放开始增加，这表明它参与了α2-巨球蛋白的受体，对D-PHI有活性，但对另外两种没有活性。生物材料。在存在胎牛血清（FBS）的竞争性蛋白结合过程中未观察到这种反应，这表明结合蛋白的排列更复杂，它们之间的相互作用以及各个生物材料的表面化学作用，与人类单核细胞相互作用时，D-PHI的低活化特性。

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