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首页> 外文期刊>Biomaterials >Multilayered, core/shell nanoprobes based on magnetic ferric oxide particles and quantum dots for multimodality imaging of breast cancer tumors
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Multilayered, core/shell nanoprobes based on magnetic ferric oxide particles and quantum dots for multimodality imaging of breast cancer tumors

机译：基于磁性三氧化二铁颗粒和量子点的多层核/壳纳米探针，用于乳腺癌肿瘤的多峰成像

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摘要

Multilayered, core/shell nanoprobes (MQQ-probe) based on magnetic nanoparticles (MNPs) and quantum dots (QDs) have been successfully developed for multimodality tumor imaging. This MQQ-probe contains Fe 3O 4 MNPs, visible-fluorescent QDs (600 nm emission) and near infrared-fluorescent QDs (780 nm emission) in multiple silica layers. The fabrication of the MQQ-probe involves the synthesis of a primer Fe 3O 4 MNPs/SiO 2 core by a reverse microemulsion method. The MQQ-probe can be used both as a fluorescent probe and a contrast reagent of magnetic resonance imaging. For breast cancer tumor imaging, anti-HER2 (human epidermal growth factor receptor 2) antibody was conjugated to the surface of the MQQ-probe. The specific binding of the antibody conjugated MQQ-probe to the surface of human breast cancer cells (KPL-4) was confirmed by fluorescence microscopy and fluorescence-activated cell sorting analysis in vitro. Due to the high tissue permeability of near-infrared (NIR) light, NIR fluorescence imaging of the tumor mice (KPL-4 cells transplanted) was conducted by using the anti-HER2 antibody conjugated MQQ-probe. In vivo multimodality images of breast tumors were successfully taken by NIR fluorescence and T 2-weighted magnetic resonance. Antibody conjugated MQQ-probes have great potential to use for multimodality imaging of cancer tumors in vitro and in vivo.
机译：基于磁性纳米颗粒（MNP）和量子点（QD）的多层核/壳纳米探针（MQQ-probe）已成功开发用于多模态肿瘤成像。该MQQ探针在多层二氧化硅层中包含Fe 3O 4 MNP，可见荧光QD（发射600 nm发射）和近红外荧光QD（发射780 nm）。 MQQ探针的制备涉及通过反向微乳液法合成底漆Fe 3O 4 MNPs / SiO 2核。 MQQ探针既可以用作荧光探针，也可以用作磁共振成像的造影剂。对于乳腺癌肿瘤成像，将抗HER2（人类表皮生长因子受体2）抗体偶联到MQQ探针的表面。通过荧光显微镜和体外荧光激活细胞分选分析，证实了缀合了抗体的MQQ-探针与人乳腺癌细胞（KPL-4）的特异性结合。由于近红外（NIR）光具有很高的组织渗透性，因此使用抗HER2抗体偶联的MQQ探针对肿瘤小鼠（移植的KPL-4细胞）进行了NIR荧光成像。通过NIR荧光和T 2加权磁共振成功拍摄了乳腺肿瘤的体内多模态图像。抗体偶联的MQQ探针在体外和体内对肿瘤的多模态成像具有巨大的潜力。

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