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首页> 外文期刊>Biomaterials >Effects of TGF-beta1 and hydrostatic pressure on meniscus cell-seeded scaffolds.
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Effects of TGF-beta1 and hydrostatic pressure on meniscus cell-seeded scaffolds.

机译：TGF-beta1和静水压力对半月​​板细胞接种支架的影响。

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The combinatorial effects of TGF-beta1 and hydrostatic pressure (HP) were investigated on meniscus cell-seeded PLLA constructs using a two-phase sequential study. The objective was to identify potentially synergistic effects of these stimuli toward enhancing the biomechanical and compositional characteristics of the engineered constructs. In Phase I, the effects of TGF-beta1 were examined on the ability of meniscus cells to produce ECM. In Phase II, meniscus cell-seeded PLLA constructs were cultured for 4 wks with a combination of TGF-beta1 and HP (10 MPa, 0 Hz or 10 MPa, 0.1 Hz). TGF-beta1 was found to increase collagen and GAG deposition in the scaffolds 15-fold and 8-fold, respectively, in Phase I. In Phase II, the combination of TGF-beta1 and 10 MPa, 0 Hz HP resulted in 4-fold higher collagen deposition (additive increase), 3-fold higher GAG deposition and enhanced compressive properties (additive and synergistic increases), when compared to the unpressurized no growth factor culture control. Though significant correlations were observed between the compressive properties (moduli and viscosity), and the GAG and collagen content of the constructs, the correlations were stronger with collagen. This study provides robust evidence that growth factors and HP can be used successfully in combination to enhance the functional properties of in vitro engineered knee meniscus constructs.
机译：使用两阶段顺序研究，研究了TGF-β1和静水压（HP）对半月形细胞接种的PLLA构建体的组合作用。目的是确定这些刺激对增强工程构建体的生物力学和组成特征的潜在协同作用。在第一阶段，研究了TGF-β1对半月板细胞产生ECM能力的影响。在阶段II中，用TGF-beta1和HP（10 MPa，0 Hz或10 MPa，0.1 Hz）的组合将半月板接种细胞的PLLA构建体培养4周。在阶段I中，发现TGF-beta1分别增加了15倍和8倍支架中胶原蛋白和GAG的沉积。在阶段II中，TGF-beta1和10 MPa，0 Hz HP的组合导致了4倍与未加压的无生长因子培养对照相比，胶原蛋白的沉积更高（增加），GAG沉积高出3倍，压缩特性提高（添加和协同增加）。尽管观察到压缩性质（模量和粘度）与构建体的GAG和胶原含量之间存在显着的相关性，但与胶原的相关性更强。这项研究提供了有力的证据，证明生长因子和HP可以成功地结合使用，以增强体外工程化的膝盖半月板构造的功能特性。

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