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首页> 外文期刊>Systematic and Applied Microbiology >Diversity and phylogeny of rhizobia associated with Desmodium spp. in Panxi, Sichuan, China
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Diversity and phylogeny of rhizobia associated with Desmodium spp. in Panxi, Sichuan, China

机译:根瘤菌与根瘤菌相关的多样性和系统发育。在中国四川攀西

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摘要

Thirty-four rhizobial isolates were obtained from root nodules of four wild Desmodium species growing in Panxi, Sichuan, China. According to the combined ARDRA and IGS-RFLP (CACAI) cluster analysis, Rhizobium, Pararhizobium and Mesorhizobium isolates outnumbered Bradyrhizobium isolates. In general, the isolates representing the same species from the same site clustered together. Furthermore, the four Desmodium species were all nodulated by more than one rhizobial species. AFLP and phenotypic analyses showed that the 34 isolates represented at least 32 distinct strains. None of the strains were found from more than one site or host, indicating a high degree of rhizobial diversity in Panxi. In the multilocus sequence analysis, the isolates were assigned to Pararhizobium giardinii, Bradyrhizobium japonicum, Mesorhizobium septentrionale, and to undescribed species of the genera Rhizobium, Bradyrhizobium and Agrobacterium. (C) 2015 Published by Elsevier GmbH.
机译:从生长在中国四川攀西的四种野生地衣草的根瘤中获得了34种根瘤菌分离株。根据结合的ARDRA和IGS-RFLP(CACAI)聚类分析,根瘤菌,副根瘤菌和中根瘤菌的分离株数超过根瘤菌。通常,来自同一地点的代表相同物种的分离株聚集在一起。此外,这四个地衣藻均由一种以上的根瘤菌属致瘤。 AFLP和表型分析表明,34个分离株代表至少32个不同的菌株。在一个以上的地点或宿主中均未发现该菌株,表明攀西地区的根瘤菌高度多样性。在多基因座序列分析中,将分离物分配给贾第寄生虫,日本慢生根瘤菌,中生根瘤菌以及根瘤菌,慢生根瘤菌和农杆菌属的未描述物种。 (C)2015由Elsevier GmbH发布。

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