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首页> 外文期刊>Symbiosis >Identification of genes involved in salt tolerance and symbiotic nitrogen fixation in chickpea rhizobium Mesorhizobium ciceri Ca181.
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Identification of genes involved in salt tolerance and symbiotic nitrogen fixation in chickpea rhizobium Mesorhizobium ciceri Ca181.

机译：鉴定鹰嘴豆根瘤菌中生根瘤菌Ca181中耐盐和共生固氮相关的基因。

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摘要

In an attempt to find the genes involved in salt tolerance of the highly adaptable chickpea rhizobium strain, Mesorhizobium ciceri Ca181, a Tn5 transposon insertion library was generated and screened to identify five mutants with inability to survive in the presence of 0.1 M NaCl. The genes disrupted in these mutants due to insertion of the transposon were identified by sequencing of Tn5 flanking sequences after inverse PCR. One of the mutants had a disruption in diguanylate cyclase gene which is involved in bacterial biofilm formation and persistence. The second mutant had a disruption in an ABC transporter membrane protein gene, which is involved in the uptake of nutrients and cellular osmoprotection. The third mutant had a disruption in a gene showing homology with rhamnulose 1-phosphate aldolase which has an important role in the central metabolism of L-rhamnulose. The fourth mutant had a disruption in a capsule synthesis gene and the fifth mutant had an insertion in an oxidoreductase gene. When these mutants were inoculated into the host chickpea plant under normal non-saline conditions, they formed symbiotic nodules but with severely reduced nitrogenase activity. Hence, it appears that bacterial ability to adapt to hyper-osmotic salt stress conditions is also important for its nitrogen fixing ability in the chickpea root nodules. Allele mining for variant forms of the identified genes in the germplasm resources of M. ciceri may help in the development of highly adaptive and efficient nitrogen fixing strains of the chickpea rhizobium.
机译：为了找到与高度适应的鹰嘴豆根瘤菌菌株Mesorhizobium ciceri Ca181的耐盐性有关的基因，生成了Tn5转座子插入文库并进行了筛选，以鉴定5个在0.1 M NaCl存在下无法存活的突变体。通过反向PCR后对Tn5侧翼序列进行测序，鉴定了由于转座子的插入而在这些突变体中破坏的基因。其中一个突变体的双鸟苷酸环化酶基因被破坏，这与细菌生物膜的形成和持久性有关。第二个突变体的ABC转运蛋白蛋白基因被破坏，这与营养吸收和细胞渗透保护有关。第三个突变体的一个基因被破坏，该基因与鼠李糖1-磷酸醛缩醛糖醛缩酶具有同源性，后者在L-鼠李糖的中央代谢中起重要作用。第四突变体的胶囊合成基因被破坏，第五突变体的氧化还原酶基因被插入。当这些突变体在正常的非盐碱条件下接种到宿主鹰嘴豆植物中时，它们形成了共生结节，但固氮酶活性却大大降低。因此，看来适应鹰嘴豆根瘤中细菌固氮能力的细菌适应高渗盐胁迫条件的能力也很重要。等位基因挖掘在M. ciceri的种质资源中鉴定出的基因的变异形式可能有助于开发鹰嘴豆根瘤菌的高度适应性和高效固氮菌株。

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