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首页> 外文期刊>Symbiosis >Thin-layer chromatographic analysis of lumichrome, riboflavin and indole acetic acid in cell-free culture filtrate of Psoralea nodule bacteria grown at different pH, salinity and temperature regimes
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Thin-layer chromatographic analysis of lumichrome, riboflavin and indole acetic acid in cell-free culture filtrate of Psoralea nodule bacteria grown at different pH, salinity and temperature regimes

机译：在不同pH，盐度和温度条件下生长的Psoralea根瘤菌无细胞培养滤液中的荧光色素，核黄素和吲哚乙酸的薄层色谱分析

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Using thin-layer chromatography, 16 bacterial isolates from root nodules of 8 different Psoralea species were quantitatively assessed for their exudation of the metabolites lumichrome, riboflavin and IAA in response to pH, salinity and temperature. Our data showed that the bacterial strains tested differed in their levels of secretion of the three metabolites. For example, strain AS2 produced significantly greater amounts of lumichrome at both pH 5.1 and 8.1, while strains RT1 and P1 produced more lumichrome per cell at only pH 8.1. Strains AP1 and RP2 also produced more riboflavin atpH 5.1 than at pH 8.1; conversely strain RT1 secreted more riboflavin at pH 8.1 than at pH 5.1. Two P. repens strains (RP1 and RP2) isolated from very saline environments close to the Indian Ocean produced significant levels of lumichrome and riboflavin at both low and high salinity treatments. However, strains AC1 and L1 (from P. aculeata and P. laxa) even produced greater amounts of lumichrome and riboflavin at higher salinity (i.e. 34.2 mM NaCl) and probably originated from naturally saline soils. In this study, high acidity and high temperature induced the synthesis and release of high levels of IAA by bacterial cells. In contrast, there was greater strain secretion of lumichrome at lower temperature (10 degrees C) than at high temperature (30 degrees C). The variations in the secretion of lumichrome, riboflavin and IAA by bacterial strains exposed to different pH, salinity and temperature regimes suggest that genes encoding these metabolites are regulated differently by the imposed environmental factors. The data from this study also suggest that natural changes of pH, salinity and/or temperature in plant rhizospheres could potentially elevate the concentrations of lumichrome, riboflavin and IAA in soils. An accumulation of these molecules in the rhizosphere would have consequences for ecosystem functioning as both lumichrome and riboflavin have been reported to act as developmental signals that affect species in all three plant, animal, and microbial kingdoms.
机译：使用薄层色谱法，定量评估了来自8种不同补骨脂类植物根瘤的16种细菌分离株响应于pH值，盐度和温度而渗出的代谢产物Luminchrome，核黄素和IAA。我们的数据表明，所测试的细菌菌株在三种代谢产物的分泌水平上有所不同。例如，菌株AS2在pH 5.1和8.1时均产生大量的荧光色素，而菌株RT1和P1仅在pH 8.1时每个细胞产生更多的荧光色素。在pH 5.1时，菌株AP1和RP2产生的核黄素也比在pH 8.1时产生的多。相反，菌株RT1在pH 8.1时比在pH 5.1时分泌更多的核黄素。从低盐度和高盐度处理中，从靠近印度洋的盐碱化环境中分离出的两种青枯菌菌株（RP1和RP2）产生了大量的发光色素和核黄素。但是，菌株AC1和L1（来自aculeata和P.laxa）甚至在较高盐度（即34.2 mM NaCl）下产生了大量的荧光染料和核黄素，并且可能源自天然盐渍土。在这项研究中，高酸度和高温诱导细菌细胞合成和释放高水平的IAA。相反，在较低温度（10摄氏度）下比在高温（30摄氏度）下发光的菌株有更多的分泌物。暴露于不同pH，盐度和温度条件下的细菌菌株在发光，核黄素和IAA分泌方面的变化表明，编码这些代谢物的基因受环境因素的调控不同。这项研究的数据还表明，植物根际中pH，盐度和/或温度的自然变化可能会提高土壤中发光色素，核黄素和IAA的浓度。这些分子在根际中的积累将对生态系统的功能产生影响，因为据报导，发光色素和核黄素都充当影响所有三个动植物界物种的发育信号。

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