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首页> 外文期刊>Pancreatology: official journal of the International Association of Pancreatology (IAP) ... [et al.] >Vigilin and enzyme expression in isolated pancreatic acini after mellitin and gamma-interferon treatment.
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Vigilin and enzyme expression in isolated pancreatic acini after mellitin and gamma-interferon treatment.

机译：蜜糖素和γ-干扰素治疗后，离体胰腺腺泡蛋白素和酶的表达。

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BACKGROUND/AIMS: Pancreatitis goes along with changes in exocrine enzyme synthesis and secretion in pancreatic acini. The multi-KH domain protein vigilin is supposed to play an important role in t-RNA trafficking especially in cells with high protein synthesis rates and may reflect the degree of stimulation of translational machinery during pathological processes. In relation to these phenomena we explored in this connection the impact of two different inflammation mediators in a system of isolated rat pancreatic acini. METHODS: Acini were prepared from male Sprague-Dawley rats by collagenase digestion and incubated with mellitin or gamma interferon. Secretion and cytosolic cell content of pancreatic trypsin and amylase as well as the expression of vigilin were determined. RESULTS: The phospholipase A(2) activator mellitin caused morphological alterations and increased release of trypsin and amylase, while vigilin expression and the intracellular content of these enzymes decreased. Gamma-interferon, a cytokine which is involved at different steps in inflammation processes, selectively inhibits the release of trypsin(ogen) while not affecting amylase secretion and vigilin expression. CONCLUSION: Mellitin as well as gamma interferon causes alterations in pancreatic enzyme secretion. Additionally, mellitin seems to influence the expressed gene pattern of pancreatic acini while interferon-gamma has no effect on protein synthesis but enzyme secretion.
机译：背景/目的：胰腺炎伴随着胰腺腺泡中外分泌酶合成和分泌的变化。多KH域蛋白守卫蛋白被认为在t-RNA转运中起着重要作用，尤其是在具有高蛋白合成率的细胞中，并且可能反映了病理过程中翻译机制的刺激程度。关于这些现象，我们在这方面探讨了两种不同的炎症介质在离体大鼠胰腺腺泡系统中的影响。方法：通过胶原酶消化法从雄性Sprague-Dawley大鼠中制备Acini，并与马来素或γ干扰素一起孵育。测定胰腺胰蛋白酶和淀粉酶的分泌和胞浆细胞含量以及维吉林的表达。结果：磷脂酶A（2）激活剂马来菌素引起形态改变，并增加了胰蛋白酶和淀粉酶的释放，而维吉林蛋白的表达和这些酶的细胞内含量均下降。 γ-干扰素是一种参与炎症过程不同步骤的细胞因子，可选择性抑制胰蛋白酶（原）的释放，同时不影响淀粉酶的分泌和维吉林的表达。结论：褪黑素以及γ干扰素引起胰腺酶分泌的改变。此外，赛璐seems似乎影响胰腺腺泡蛋白的表达基因模式，而干扰素-γ对蛋白质合成没有影响，但对酶的分泌有影响。

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