...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pancreatology: official journal of the International Association of Pancreatology (IAP) ... [et al.] >Gene expression signature of advanced pancreatic ductal adenocarcinoma using low density array on endoscopic ultrasound-guided fine needle aspiration samples.
【24h】

Gene expression signature of advanced pancreatic ductal adenocarcinoma using low density array on endoscopic ultrasound-guided fine needle aspiration samples.

机译：使用低密度阵列在内镜超声引导下细针穿刺样本上进行晚期胰腺导管腺癌的基因表达签名。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of this study was to investigate the clinical feasibility and utility of low-density array analysis on samples obtained from endoscopic ultrasound-guided fine needle aspiration biopsy in locally advanced and/or metastatic pancreatic ductal adenocarcinoma and chronic pancreatitis.In this prospective multicenter study, we quantified candidate gene expression in biopsies sampled from 44 locally advanced and/or metastatic pancreatic carcinoma and from 17 pseudotumoural chronic pancreatitis using dedicated low-density array microfluidic plates.We first demonstrated that 18S gene expression is stable and comparable in normal pancreas and pancreatic cancer tissues. Next, we found that eight genes (S100P, PLAT, PLAU, MSLN, MMP-11, MMP-7, KRT7, KRT17) were significantly over expressed in pancreatic cancer samples when compared to pseudotumoural chronic pancreatitis (p value ranging from 0.0007 to 0.0215): Linear discriminative analysis identified S100P, PLAT, MSLN, MMP-7, KRT7 as highly explicative variables. The area under receiver operating curve establishes the clinical validity of the potential diagnostic markers identified in this study (values ranging from 0.69 to 0.76). In addition, combination of S100P and KRT7 gave better diagnosis performances (Area Under Receiver Operating Curve 0.81, sensitivity 81%, specificity 77%).We demonstrate that molecular studies on EUS-guided FNA material are feasible for the identification and quantification of markers in PDAC patients diagnosed with non-resectable tumours. Using low-density array, we isolated a molecular signature of advanced pancreatic carcinoma including mostly cancer invasion-related genes. This work stems for the use of novel biomarkers for the molecular diagnosis of patient with solid pancreatic masses.
机译：这项研究的目的是探讨低密度阵列分析对内镜超声引导下的细针穿刺活检获得的样品在局部晚期和/或转移性胰腺导管腺癌和慢性胰腺炎中的临床可行性和实用性。 ，我们使用专用的低密度阵列微流板对44例局部晚期和/或转移性胰腺癌和17例假肿瘤慢性胰腺炎的活检样本中的候选基因表达进行了量化。我们首先证明18S基因在正常胰腺和胰腺中表达稳定且具有可比性癌组织。接下来，我们发现与假瘤性慢性胰腺炎相比，胰腺癌样品中八个基因（S100P，PLAT，PLAU，MSLN，MMP-11，MMP-7，KRT7，KRT17）显着过表达（p值在0.0007至0.0215之间） ）：线性判别分析确定S100P，PLAT，MSLN，MMP-7，KRT7为高度解释变量。接收器工作曲线下方的区域确定了本研究中确定的潜在诊断标志物的临床有效性（值范围为0.69至0.76）。此外，S100P和KRT7的组合具有更好的诊断性能（受体工作曲线下面积0.81，灵敏度81％，特异性77％）。 PDAC患者被诊断患有不可切除的肿瘤。使用低密度阵列，我们分离出了晚期胰腺癌的分子特征，其中主要包括与癌症侵袭相关的基因。这项工作源于使用新型生物标记物对固体胰腺肿块患者进行分子诊断。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号