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首页> 外文期刊>Parasitology >Long-term passage of tachyzoites in tissue culture can attenuate virulence of Neospora caninum in vivo.
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Long-term passage of tachyzoites in tissue culture can attenuate virulence of Neospora caninum in vivo.

机译：速殖子在组织培养中的长期传播可以减弱犬新孢子虫的体内毒力。

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摘要

To determine whether prolonged in vitro passage would result in attenuation of virulence in vivo, Neospora caninum tachyzoites were passaged for different lengths of time in vitro and compared for their ability to cause disease in mice. Groups of Balb/c mice were inoculated intraperitoneally with 5 x 10(6) or 1 x 10(7) of low-passage or high-passage N. caninum tachyzoites. The mice were monitored for changes in their demeanour and body weight, and were culled when severe clinical symptoms of murine neosporosis were observed. Mice inoculated with the high-passage parasites survived longer (P<0.05), and showed fewer clinical symptoms of murine neosporosis, compared to the mice receiving the low-passage parasites. The parasite was detected in the brains of inoculated mice using immunohistochemistry and ITS1 PCR. Tissue cysts containing parasites were seen in mice inoculated with both low-passage and high-passage parasites. When the in vitro growth rates of the parasites were compared, the high-passage parasites initially multiplied more rapidly (P<0.001) than the low-passage parasites, suggesting that the high-passage parasites had become more adapted to tissue culture. These results would suggest that it is possible to attenuate the virulence of N. caninum tachyzoites in mice through prolonged in vitro passage.
机译：为了确定延长的体外传代是否会导致体内毒力的减弱，将新孢子虫速殖子在体外传代不同的时间长度，并比较它们在小鼠中引起疾病​​的能力。将Balb / c小鼠组腹膜内接种5 x 10（6）或1 x 10（7）的低通道或高通道N. caninum速殖子。监测小鼠的行为和体重变化，并在观察到鼠新孢子虫病的严重临床症状时将其剔除。与接受低通道寄生虫的小鼠相比，接种高通道寄生虫的小鼠存活时间更长（P <0.05），并且鼠新孢子虫病的临床症状更少。使用免疫组织化学和ITS1 PCR在接种小鼠的大脑中检测到了寄生虫。在接种了低通道和高通道寄生虫的小鼠中均发现了含有寄生虫的组织囊肿。当比较寄生虫的体外生长速率时，高通道寄生虫的繁殖开始比低通道寄生虫繁殖更快（P <0.001），这表明高通道寄生虫变得更加适应组织培养。这些结果表明，有可能通过延长体外传代来减轻小鼠中猪新孢子虫速殖子的毒性。

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