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首页> 外文期刊>Parasitology >Assessment of cathepsin D and L-like proteinases of poultry red mite, Dermanyssus gallinae (De Geer), as potential vaccine antigens
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Assessment of cathepsin D and L-like proteinases of poultry red mite, Dermanyssus gallinae (De Geer), as potential vaccine antigens

机译：评估家禽红螨Dermanyssus gallinae（De Geer）的组织蛋白酶D和L样蛋白酶作为潜在的疫苗抗原

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Vaccination is a feasible strategy for controlling the haematophagous poultry red mite Dermanyssus gallinae. A cDNA library enriched for genes upregulated after feeding was created to identify potential vaccine antigens. From this library, a gene (Dg-CatD-1) encoding a 383 amino acid protein (Dg-CatD-1) with homology to cathepsin D lysosomal aspartyl proteinases was identified as a potential vaccine candidate. A second gene (Dg-CatL-1) encoding a 341 amino acid protein (Dg-CatL-1) with homology to cathepsin L cysteine proteinases was also selected for further study. IgY obtained from naturally infested hens failed to detect Dg-CatD-1 suggesting that it is a concealed antigen. Conversely, Dg-CatL-1 was detected by IgY derived from natural-infestation, indicating that infested hens are exposed to Dg-CatL-1. Mortality rates 120 h after mites had been fed anti-Dg-CatD-1 were significantly higher than those fed control IgY (PF<0·01). In a survival analysis, fitting a proportional hazards model to the time of death of mites, anti-Dg-CatD-1 and anti-Dg-CatL-1 IgY had 4·42 and 2·13 times higher risks of dying compared with controls (PF<0·05). Dg-CatD-1 and L-1 both have potential as vaccine antigens as part of a multi-component vaccine and have the potential to be improved as vaccine antigens using alternative expression systems.
机译：接种疫苗是控制食血性家禽红螨Dermanyssus gallinae的可行策略。建立了一个富含进食后上调基因的cDNA文库，以鉴定潜在的疫苗抗原。从该文库中，与组织蛋白酶D溶酶体天冬氨酰蛋白酶同源的，编码383个氨基酸的蛋白质（Dg-CatD-1）的基因（Dg-CatD-1）被确定为潜在的疫苗候选物。还选择了第二个基因（Dg-CatL-1），该基因编码与组织蛋白酶L半胱氨酸蛋白酶具有同源性的341个氨基酸蛋白（Dg-CatL-1）。从自然出没的母鸡获得的IgY无法检测到Dg-CatD-1，表明它是一种隐蔽的抗原。相反地​​，Dg-CatL-1被自然侵染的IgY检测到，表明被感染的母鸡暴露于Dg-CatL-1。饲喂抗Dg-CatD-1的螨虫120小时后的死亡率显着高于对照IgY的死亡率（PF <0·01）。在生存分析中，对螨的死亡时间拟合比例风险模型，抗Dg-CatD-1和抗Dg-CatL-1 IgY的死亡风险比对照组高4·42和2·13倍（PF <0·05）。 Dg-CatD-1和L-1都具有作为疫苗抗原的潜力，可作为多组分疫苗的一部分，并且具有使用替代表达系统作为疫苗抗原的潜力。

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