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首页> 外文期刊>Parasitology >Cloning and molecular analysis of the aspartic protease Sc-ASP110 gene transcript in Steinernema carpocapsae
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Cloning and molecular analysis of the aspartic protease Sc-ASP110 gene transcript in Steinernema carpocapsae

机译：腕果Steinernema carpocapsae中天冬氨酸蛋白酶Sc-ASP110基因转录物的克隆和分子分析

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SUMMARY Many protease genes have previously been shown to be involved in parasitism and in the development of Steinernema carpocapsae, including a gene predicted to encode an aspartic protease, Sc-ASP110, which was cloned and was analysed in this study. A cDNA encoding Sc-ASP110 was cloned based on an expressed sequence tag (EST) fragment from our EST library. The full-length cDNA of Sc-ASP110 consists of 1112 nucleotides with a catalytic aspartic domain (aa18-337). The putative 341 amino acid residues have a calculated molecular mass of 37·1 kDa and a theoretical pI of 4·7. BLASTp analysis of the Sc-ASP110 amino acid sequence showed 45-77% amino acid sequence identity to parasitic and non-parasitic nematode aspartic proteases. An expression analysis showed that the sc-asp110 gene was upregulated during the late parasitic stage, L4, and 24 h after induction of in vitro nematodes. A sequence comparison revealed that Sc-ASP110 was a member of an aspartic protease family; additionally, a phylogenetic analysis indicated that Sc-ASP110 was clustered with the closely related nematode Steinernema feltiae. In situ hybridization showed that sc-asp110 was expressed in the body walls of dorsal cells. The upregulated Sc-ASP110 expression revealed that this protease could play a role in the late parasitic process. In this study, we have cloned and analysed the gene transcript of Sc-ASP110 in S. carpocapsae.
机译：发明内容以前已经显示出许多蛋白酶基因参与寄生虫病和炭疽Steinernema carpocapsae的发展，包括预测编码天冬氨酸蛋白酶Sc-ASP110的基因，该基因已被克隆并在本研究中进行了分析。基于来自我们EST文库的表达序列标签（EST）片段，克隆了编码Sc-ASP110的cDNA。 Sc-ASP110的全长cDNA由1112个具有催化天冬氨酸结构域（aa18-337）的核苷酸组成。假定的341个氨基酸残基的计算分子量为37·1 kDa，理论pI为4·7。对Sc-ASP110氨基酸序列的BLASTp分析显示与寄生和非寄生线虫天冬氨酸蛋白酶具有45-77％的氨基酸序列同一性。表达分析表明，sc-asp110基因在寄生虫晚期，L4和诱导体外线虫后24 h上调。序列比较表明，Sc-ASP110是天冬氨酸蛋白酶家族的成员。此外，系统发育分析表明，Sc-ASP110与紧密相关的线虫Steinernema thinkiae聚集在一起。原位杂交表明sc-asp110在背细胞体壁中表达。 Sc-ASP110表达上调表明该蛋白酶可能在后期的寄生虫过程中起作用。在这项研究中，我们已经克隆和分析了S. carpocapsae中Sc-ASP110的基因转录本。

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