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首页> 外文期刊>Parasitology >Evidence of the three main clonal Toxoplasma gondii lineages from wild mammalian carnivores in the UK
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Evidence of the three main clonal Toxoplasma gondii lineages from wild mammalian carnivores in the UK

机译：英国野生哺乳动物食肉动物的三种主要克隆弓形虫谱系的证据

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摘要

Toxoplasma gondii is a zoonotic pathogen defined by three main clonal lineages (types I, II, III), of which type II is most common in Europe. Very few data exist on the prevalence and genotypes of T. gondii in the UK. Wildlife can act as sentinel species for T. gondii genotypes present in the environment, which may subsequently be transmitted to livestock and humans. DNA was extracted from tissue samples of wild British carnivores, including 99 ferrets, 83 red foxes, 70 polecats, 65 mink, 64 badgers and 9 stoats. Parasite DNA was detected using a nested ITS1 PCR specific for T. gondii, PCR positive samples were subsequently genotyped using five PCR-RFLP markers. Toxoplasma gondii DNA was detected within all these mammal species and prevalence varied from 6·0 to 44·4% depending on the host. PCR-RFLP genotyping identified type II as the predominant lineage, but type III and type I alleles were also identified. No atypical or mixed genotypes were identified within these animals. This study demonstrates the presence of alleles for all three clonal lineages with potential for transmission to cats and livestock. This is the first DNA-based study of T. gondii prevalence and genotypes across a broad range of wild British carnivores.
机译：弓形虫是一种人畜共患病原体，由三种主要的克隆谱系（I，II，III型）定义，其中II型在欧洲最为常见。在英国，关于弓形虫的流行和基因型的数据很少。野生动物可以作为环境中存在的弓形虫基因型的定点物种，随后可能传播给牲畜和人类。从野生英国食肉动物的组织样品中提取DNA，包括99只雪貂，83只赤狐，70只猫，65只貂，64只rs和9沙特。使用嵌套的特异于弓形虫的ITS1 PCR检测寄生虫DNA，随后使用5个PCR-RFLP标记对PCR阳性样品进行基因分型。在所有这些哺乳动物物种中均检测到弓形虫DNA，根据宿主的不同，患病率从6·0到44·4％不等。 PCR-RFLP基因分型确定II型为主要谱系，但也鉴定出III型和I型等位基因。在这些动物中未鉴定出非典型或混合基因型。这项研究表明所有三个克隆谱系均存在等位基因，并有可能传播给猫和家畜。这是首个基于DNA的弓形虫流行率和基因型在广泛的野生英国食肉动物中的研究。

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