...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Parasitology >Temporal dynamics of spore release of the crayfish plague pathogen from its natural host, American spiny-cheek crayfish (Orconectes limosus), evaluated by transmission experiments
【24h】

Temporal dynamics of spore release of the crayfish plague pathogen from its natural host, American spiny-cheek crayfish (Orconectes limosus), evaluated by transmission experiments

机译：通过传播实验评估了小龙虾鼠疫病原体从其天然宿主美国多刺小龙虾（Orconectes limosus）的孢子释放的时间动态

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

SUMMARY The crayfish plague pathogen, Aphanomyces astaci, is one of the most serious threats to indigenous European crayfish species. The North American invasive spiny-cheek crayfish, Orconectes limosus, is an important source of this pathogen in central and western Europe. We evaluated potential changes in A. astaci spore release rate from infected individuals of this species by experiments investigating the pathogen transmission to susceptible noble crayfish, Astacus astacus. We filtered defined volumes of water regularly to quantify spore concentration, and sampled crayfish tissues at the end of the experiment. The filters and tissues were then tested for the presence of A. astaci DNA by species-specific quantitative PCR. Additionally, we tested the efficiency of horizontal transmission to apparently uninfected O. limosus. The experiments confirmed that A. astaci can be transmitted to susceptible crayfish during intermoult periods, and that the pathogen was more frequently detected in noble crayfish recipients than in American ones. The pathogen spore concentrations substantially varied in time, and significantly increased during moulting of infected hosts. Our study strengthens the evidence that although the likelihood of crayfish plague transmission by water transfer from localities with infected American crayfish might increase when these are moulting or dying, no time-periods can be proclaimed safe. ? Cambridge University Press 2013.
机译：小结小龙虾鼠疫病原体Aphanomyces astaci是对欧洲本土小龙虾物种的最严重威胁之一。北美入侵性有刺颊小龙虾Orconectes limosus是中欧和西欧这种病原的重要来源。我们通过调查病原体向易感贵族小龙虾（Astacus astacus）的病原体传播的实验，评估了该物种受感染个体中A. astaci孢子释放速率的潜在变化。我们定期过滤一定体积的水以定量孢子浓度，并在实验结束时对小龙虾组织进行采样。然后通过物种特异性定量PCR测试滤器和组织中是否存在黄曲霉DNA。此外，我们测试了水平传播对显然未感染的金黄色葡萄球菌的效率。实验证实，黄曲霉可以在成年期传播给易感的小龙虾，并且在高贵的小龙虾接受者中检出病原体的频率比美国的小。病原体孢子浓度随时间变化很大，并且在被感染宿主蜕皮期间显着增加。我们的研究加强了以下证据：尽管在成年或死亡期间，受感染的美国小龙虾从受感染小龙虾的地方通过调水传播小龙虾鼠疫的可能性可能会增加，但没有任何时间可以说是安全的。 ？剑桥大学出版社，2013年。

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号