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首页> 外文期刊>Parasitology >Immunological characterization of a chimeric form of Schistosoma mansoni aquaporin in the murine model.
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Immunological characterization of a chimeric form of Schistosoma mansoni aquaporin in the murine model.

机译：曼氏血吸虫水通道蛋白的嵌合形式在鼠模型中的免疫学表征。

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Aquaporin (SmAQP) is the most abundant transmembrane protein in the tegument of Schistosoma mansoni. This protein is expressed in all developmental stages and seems to be essential in parasite survival since it plays a crucial role in osmoregulation, nutrient transport and drug uptake. In this study, we utilized the murine model to evaluate whether this protein was able to induce protection against challenge infection with S. mansoni cercariae. A chimeric (c) SmAQP was formulated with Freund's adjuvant for vaccination trial and evaluation of the host's immune response was performed. Our results demonstrated that immunization with cSmAQP induced the production of high levels of specific anti-cSmAQP IgG antibodies and a Th1/Th17 type of immune response characterized by IFN-γ, TNF-α and IL-17 cytokines. However, vaccination of mice with cSmAQP failed to reduce S. mansoni worm burden and liver pathology. Finally, we were unable to detect humoral immune response anti-cSmAQP in the sera of S. mansoni-infected human patients. Our results lead us to believe that SmAQP, as formulated in this study, may not be a good target in the search for an anti-schistosomiasis vaccine.
机译：水通道蛋白（SmAQP）是曼氏血吸虫中最丰富的跨膜蛋白。该蛋白在所有发育阶段均表达，并且似乎在寄生虫生存中必不可少，因为它在渗透调节，营养物质运输和药物吸收中起着至关重要的作用。在这项研究中，我们利用鼠模型来评估该蛋白是否能够诱导针对曼氏葡萄球菌的挑战性感染的保护作用。嵌合（c）SmAQP与弗氏佐剂一起配制用于疫苗接种试验，并评估宿主的免疫反应。我们的结果表明，用cSmAQP免疫可诱导产生高水平的特异性抗cSmAQP IgG抗体，并产生以IFN-γ，TNF-α和IL-17细胞因子为特征的Th1 / Th17型免疫应答。但是，用cSmAQP接种小鼠未能减轻曼氏沙门氏菌蠕虫的负担和肝脏病理。最后，我们无法在曼氏链球菌感染人类患者的血清中检测到抗cSmAQP的体液免疫反应。我们的结果使我们相信，本研究中制定的SmAQP可能不是寻找抗血吸虫病疫苗的良好目标。

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