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首页> 外文期刊>Parasitology >The interaction between the amoeba Balamuthia mandrillaris and extracellular matrix glycoproteins in vitro.
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The interaction between the amoeba Balamuthia mandrillaris and extracellular matrix glycoproteins in vitro.

机译：变形虫变形虫Balamuthia mandrillaris与细胞外基质糖蛋白的体外相互作用。

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Balamuthia mandrillaris, a soil amoeba, is the causative agent of Balamuthia granulomatous amoebic encephalitis, a life-threatening brain infection. This amoeba is acquired from contaminated soil and may enter the host through cutaneous lesions or through nasal passages, migrating to the lungs or brain. During invasion, B. mandrillaris has access to components of the extracellular matrix (ECM) of the host. Therefore, we investigated the interaction of B. mandrillaris with 3 ECM glycoproteins (collagen-I, fibronectin and laminin-1) that are encountered in host connective tissues and at the basal lamina. Using optical microscopy, amoeba association on ECM-coated surfaces was examined. Binding of amoebae on laminin was greater than that on collagen or fibronectin. Laminin-adhered B. mandrillaris exhibited elongated and spread forms, distinctive from those observed for amoebae on a plastic surface. Collagen and fibronectin-adhered B. mandrillaris presented elongated shapes with cellular expansions. Bindingto collagen, fibronectin, or laminin was inhibited when amoebae were pre-treated with sialic acid. Treatment with galactose resulted in diminished binding of amoebae on laminin, while mannose increased binding in all coating conditions tested. Dependence of divalent cations on amoeba binding was demonstrated for laminin-amoeba interaction. Collectively, the results indicate that B. mandrillaris recognizes specific glycoproteins of the mammalian extracellular matrix.
机译：土壤变形虫阿米巴虫（Balamuthia mandrillaris）是Bal虫肉芽肿性阿米巴性脑炎的病原体，它是威胁生命的脑部感染。该变形虫是从受污染的土壤中获取的，可能通过皮肤损伤或鼻腔通道进入宿主，并迁移到肺部或大脑。在入侵过程中，曼氏芽孢杆菌可接触宿主的细胞外基质（ECM）的成分。因此，我们调查了B. mandrillaris与3种ECM糖蛋白（胶原蛋白I，纤连蛋白和层粘连蛋白1）的相互作用，这些蛋白在宿主结缔组织和基底层中遇到。使用光学显微镜，检查在ECM涂层表面上的变形虫缔合。变形虫在层粘连蛋白上的结合大于在胶原蛋白或纤连蛋白上的结合。粘附有层粘连蛋白的曼氏芽孢杆菌表现出伸长和散布形式，这与在塑料表面上的变形虫观察到的形式不同。胶原蛋白和纤连蛋白粘附的曼氏芽孢杆菌呈现出细长的形状，并带有细胞膨胀。当用唾液酸预处理变形虫时，与胶原蛋白，纤连蛋白或层粘连蛋白的结合被抑制。半乳糖处理导致变形虫在层粘连蛋白上的结合减少，而甘露糖在所有测试的包被条件下均增加了结合。层粘连蛋白-阿米巴相互作用证明了二价阳离子对变形虫结合的依赖性。总体而言，结果表明，曼氏芽孢杆菌可识别哺乳动物细胞外基质的特定糖蛋白。

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