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首页> 外文期刊>Parasitology >Recombinant protein Bd37 protected gerbils against heterologous challenges with isolates of Babesia divergens polymorphic for the bd37 gene.
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Recombinant protein Bd37 protected gerbils against heterologous challenges with isolates of Babesia divergens polymorphic for the bd37 gene.

机译：重组蛋白Bd37保护了沙鼠免受异源性攻击，其分离株对bd37基因具有多态性。

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摘要

The Bd37gene encoding for a glycosyl-phosphatidyl-inositol anchored protein of Babesia divergens displays genetic polymorphisms among isolates. Five major polymorphic groups (clades) were shown by PCR-RFLP among different B. divergens isolates. Each group has been characterized according to a reference Bd37 gene (Rouen87, W8843, Y5, 6303E and 1705B). Recombinant (GST fusion) protein (Bd37r) expressed from the Bd37 gene, was used as antigen in a saponin-based formulation and was able to protect gerbils, after 2 injections at low dose vaccine concentration (1 mug per dose), against a virulent challenge with the B. divergens Rouen87 isolate. In spite of polymorphism of Bd37 gene, Bd37r induced complete immunoprotection against challenges with each of the 5 reference isolate groups defined by PCR-RFLP.
机译：编码Babesia divergens的糖基-磷脂酰-肌醇锚定蛋白的Bd37基因在分离株之间显示出遗传多态性。通过PCR-RFLP在不同的B. divergens分离物中显示了五个主要的多态组（进化枝）。每组均已根据参考Bd37基因（Rouen87，W8843，Y5、6303E和1705B）进行了表征。从Bd37基因表达的重组（GST融合）蛋白（Bd37r）在基于皂苷的配方中用作抗原，并且在低剂量疫苗浓度（每剂1杯）注射2次后，能够保护沙鼠免受强毒用B. divergens Rouen87分离菌挑战。尽管Bd37基因具有多态性，但Bd37r仍可通过PCR-RFLP定义的5个参考分离株中的每一个诱导针对攻击的完全免疫保护。

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