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首页> 外文期刊>Parasitology >Immobilization antibodies of tiger puffer Takifugu rubripes induced by i.p. injection against monogenean Heterobothrium okamotoi oncomiracidia do not prevent the infection.
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Immobilization antibodies of tiger puffer Takifugu rubripes induced by i.p. injection against monogenean Heterobothrium okamotoi oncomiracidia do not prevent the infection.

机译：i.p.诱导的老虎河豚Takifugu rubripes的固定化抗体。注射针对单基因的冈本氏异形疱疹性酸尿症不能预防感染。

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We examined whether infection by the monogenean Heterobothrium okamotoi induces production of specific antibodies against oncomiracidia and their cilia, larvae on the gills, and adults on the branchial cavity wall of tiger puffer Takifugu rubripes. We also investigated whether specific antibody production participates in acquired protection against H. okamotoi. Sera from persistently infected fish immobilized H. okamotoi oncomiracidia 89 days after exposure and antibody levels (measured by enzyme-linked immunosorbent assays) in the sera against oncomiracidia and their cilia increased compared with sera from control (naive) fish. Antibody levels in these sera against the larvae and adult stages did not increase. The number of H. okamotoi on persistently infected fish was significantly lower than for control fish (P<0.05) when persistently infected fish and control fish were exposed to oncomiracidia in the same tank. Thus tiger puffer produced specific antibodies against oncomiracidia and their cilia, and acquired partial protection against H. okamotoi. Intraperitoneal injection of proteins of sonicated oncomiracidia or their cilia with an adjuvant also produced oncomiracidium agglutination antibodies in sera from tiger puffer; the antibody levels in these sera against oncomiracidia and their cilia increased compared with sera from control fish (injection of BSA with an adjuvant) at 14, 44, and 75 days after the booster immunization. However, in a parasite challenge at 54-58 days after the booster immunization, the infection levels of fish immunized with parasites of sonicated oncomiracidia or their cilia were the same as the control fish. Western blot showed that sera from persistently infected fish and fish immunized with sonicated oncomiracidia or their cilia recognized similar antigenic bands, suggesting that tiger puffer tends to react against these antigens compared with other antigens. These results indicated that specific antibodies against these cilia and oncomiracidia induced by i.p. injection donot prevent H. okamotoi infection.
机译：我们检查了由单基因的冈本氏杂杆菌引起的感染是否诱导产生了针对鬼臼酸及其纤毛，the上的幼虫以及adults河豚T的wall腔壁上的成虫的特异性抗体。我们还调查了特定抗体的生产是否参与了针对冈本氏菌的获得性保护。暴露持续感染的鱼的血清在暴露89天后固定了冈本氏嗜酸杆菌，其血清抗猴酸和其纤毛的抗体水平（通过酶联免疫吸附法测定）与对照（幼稚）鱼的血清相比有所增加。这些血清中针对幼虫和成年阶段的抗体水平并未增加。当持续感染的鱼类和对照鱼类在同一罐中暴露于粪酸时，持续感染的鱼类中的冈本氏菌数量显着低于对照鱼类（P <0.05）。因此，老虎河豚产生了针对鬼臼酸及其纤毛的特异性抗体，并获得了针对冈本氏菌的部分保护。腹膜内注射佐剂的经超声处理的oniriracidia或其纤毛蛋白也可在老虎河豚的血清中产生oniriracidium凝集抗体。在加强免疫后第14天，第44天和第75天，与对照鱼（注射BSA和佐剂）的血清相比，这些血清中的针对甲虫酸及其纤毛的抗体水平有所提高。但是，在加强免疫后第54-58天进行了一次寄生虫攻击，用超声感染的Oncomiracidia寄生虫或其纤毛免疫的鱼的感染水平与对照鱼相同。 Western印迹表明，持续感染的鱼和经超声刀切酸病或纤毛免疫的鱼的血清识别出相似的抗原谱带，这表明与其他抗原相比，河豚倾向于与这些抗原反应。这些结果表明，由腹膜内注射诱导的针对这些纤毛和癌痛的特异性抗体。注射不能预防冈本氏菌感染。

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