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首页> 外文期刊>Parasitology >Characterization of a novel spore wall protein NbSWP16 with proline-rich tandem repeats from Nosema bombycis (microsporidia)
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Characterization of a novel spore wall protein NbSWP16 with proline-rich tandem repeats from Nosema bombycis (microsporidia)

机译：新型孢子壁蛋白NbSWP16的特征与Nosema bombycis（microsporidia）富含脯氨酸的串联重复序列

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摘要

Nosema bombycis, a pathogen of silkworm pebrine, is an obligate unicellular eukaryotic parasite. It is reported that the spore wall proteins have essential functions in the adherence and infection process of microsporidia. To date, the information related to spore wall proteins from microsporidia is still limited. Here, a 44 kDa spore wall protein NbSWP16 was characterized in N. bombycis. In NbSWP16, a 25 amino acids signal peptide and 3 heparin binding motifs were predicted. Interestingly, a region that contains 3 proline-rich tandem repeats lacking homology to any known protein was also present in this protein. The immunofluorescence analysis (IFA) demonstrated that distinct fluorescent signals were detected both on the surface of mature spores and the germinated spore coats. Immunolocation by electron microscopy revealed that NbSWP16 localized on the exospore regions. Finally, spore adherence analysis indicated that spore adherence to host cell was decreased more than 20% by anti-NbSWP16 blocking compared with the negative control in vitro. In contrast with anti-NbSWP16, no remarkable decrement inhibition was detected when antibodies of NbSWP16 and NbSWP5 were used simultaneously. Collectively, these results suggest that NbSWP16 is a new exospore protein and probably be involved in spore adherence of N. bombycis.
机译：Nosema bombycis，一种蚕白粉病的病原体，是专性的单细胞真核寄生虫。据报道，孢子壁蛋白在小孢子虫的粘附和感染过程中具有重要功能。迄今为止，与来自小孢子虫的孢子壁蛋白有关的信息仍然有限。在这里，在猪笼草中鉴定出44kDa的孢子壁蛋白NbSWP16。在NbSWP16中，预测了25个氨基酸的信号肽和3个肝素结合基序。有趣的是，该蛋白质中还存在一个含有3个富含脯氨酸的串联重复序列的区域，该重复序列与任何已知蛋白质缺乏同源性。免疫荧光分析（IFA）表明，在成熟孢子表面和发芽孢子外壳上均检测到不同的荧光信号。电子显微镜的免疫定位显示NbSWP16定位在外孢子区域。最后，孢子粘附分析表明，与阴性对照相比，抗NbSWP16阻断使孢子对宿主细胞的粘附降低了20％以上。与抗NbSWP16相比，当同时使用NbSWP16和NbSWP5抗体时，未检测到明显的减量抑制作用。总的来说，这些结果表明NbSWP16是一种新的外孢子蛋白，可能参与了N. bombycis的孢子粘附。

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