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首页> 外文期刊>Parasitology >Burden of major diarrheagenic protozoan parasitic co-infection among amoebic dysentery cases from North East India: a case report
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Burden of major diarrheagenic protozoan parasitic co-infection among amoebic dysentery cases from North East India: a case report

机译：印度东北部阿米巴痢疾病例中主要的腹泻性原生动物寄生虫共感染负担：一例病例报告

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摘要

Intestinal diarrheagenic polyparasitic infections are among the major public health concerns in developing countries. Here we examined stool specimens by microscopy, DNA dot blot and polymerase chain reaction (PCR) to evaluate the co-infection of four principal protozoans among amoebic dysentery cases from Northeast Indian population. The multiplex PCR confirmed Entamoeba histolytica (8.1%), Entamoeba dispar (4.8%) and mixed infection of both the parasites (3.4%) in 68 of 356 stool specimens that were positive in microscopy and/or HMe probe based DNA dot blot screening. The prevailing parasite that co-exists with E. histolytica was Giardia duodenalis (34.1%), followed by Enterocytozoon bieneusi (22.0%), Cryptosporidium parvum (14.6%) and Cyclospora cayetanensis (7.3%, P = 0.017). Symptomatic participants (odds ratio (OR) = 4.07; 95% confidence interval (CI) = 1.06, 15.68; P = 0.041), monsoon season (OR = 7.47; 95% CI = 1.40, 39.84; P = 0.046) and participants with family history of parasitic infection (OR = 4.50; 95% CI = 1.16, 17.51; P = 0.030) have significant association with overall co-infection rate. According to molecular consensus, comprehensive microscopy yielded 3.4% (12/356) false-negative and 7.6% (27/356) false-positive outcome, suggesting an improved broad-spectrum PCR-based diagnostic is required to scale down the poor sensitivity and specificity as well as implementation of integrated control strategy.
机译：肠道腹泻性多寄生虫感染是发展中国家的主要公共卫生问题。在这里，我们通过显微镜，DNA点印迹和聚合酶链反应（PCR）检查了粪便标本，以评估印度东北部人群的阿米巴痢疾病例中四种主要原生动物的共同感染。多重PCR证实了356份粪便标本中的68份在显微镜下和/或基于HMe探针的DNA斑点印迹筛查中呈阳性，从而证实了组织变形杆菌（8.1％），变形杆菌（4.8％）和两种寄生虫（3.4％）的混合感染。与溶组织性大肠杆菌共存的主要寄生虫是十二指肠虫（Giardia duodenalis）（34.1％），其次是比氏肠杆菌（26.6％），小隐隐孢子虫（14.6％）和毛孢环孢菌（7.3％，P = 0.017）。有症状的参与者（赔率（OR）= 4.07; 95％的置信区间（CI）= 1.06，15.68; P = 0.041），季风季节（OR = 7.47; 95％CI = 1.40，39.84; P = 0.046）和参与者寄生虫感染的家族史（OR = 4.50; 95％CI = 1.16，17.51; P = 0.030）与总体合并感染率有显着相关性。根据分子共识，综合显微镜检查可得出3.4％（12/356）的假阴性结果和7.6％（27/356）的假阳性结果，表明需要改进的基于广谱PCR的诊断方法以降低灵敏度和特异性以及综合控制策略的实施。

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