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首页> 外文期刊>Parasitology >In vitro cultivation of Hematodinium sp isolated from Atlantic snow crab, Chionoecetes opilio: partial characterization of late developmental stages
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In vitro cultivation of Hematodinium sp isolated from Atlantic snow crab, Chionoecetes opilio: partial characterization of late developmental stages

机译：从大西洋雪蟹Chionoecetes opilio分离到的Hematodinium sp的体外培养：后期发育阶段的部分表征

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摘要

Hematodinium is a parasitic dinoflagellate of numerous crustacean species, including the economically important Atlantic snow crab, Chionoecetes opilio. The parasite was cultured in vitro in modified Nephrops medium at 0 degrees C and a partial characterization of the life stages was accomplished using light and transmission electron microscopy (TEM). In haemolymph from heavily infected snow crabs two life stages were detected; amoeboid trophonts and sporonts. During in vitro cultivation, several Hematodinium sp. life stages were observed: trophonts, clump colonies, sporonts, arachnoid sporonts, sporoblasts and dinospores. Cultures initiated with sporonts progressed to motile dinospores; however, those initiated with amoeboid trophonts proliferated, but did not progress or formed schizont-like stages which were senescent artefacts. Plasmodial stages were associated with both trophonts and sporonts and could be differentiated by the presence of trichocysts on TEM. Macrodinospores were observed but not microdinospores; likely due to the low number of Hematodinium sp. cultures that progressed to the dinospore stage. No early life stages including motile filamentous trophonts or gorgonlocks were observed as previously noted in Hematodinium spp. from other crustacean hosts. All Hematodinium sp. life stages contained autofluorescent, membrane-bound electron dense granules that appeared to degranulate or be expelled from the cell during in vitro cultivation.
机译：血红素是许多甲壳类动物的寄生鞭毛虫，包括经济上重要的大西洋雪蟹Chionoecetes opilio。将寄生虫在改良的Nephrops培养基中于0摄氏度体外培养，并通过光镜和透射电子显微镜（TEM）对生命阶段进行部分表征。在严重感染的雪蟹的血淋巴中，发现了两个生命阶段。变形虫的刺和孢子。在体外培养过程中，有几个Hematodinium sp。观察到生命的各个阶段：小节，丛生菌落，孢子虫，蛛网膜孢子，孢子母细胞和恐龙孢子。从孢子开始的培养发展为能动的恐龙孢子。然而，那些以变形虫细针开始的细胞增殖了，但是没有进展或形成像裂殖体一样的阶段，这是衰老的假象。浆膜发育阶段与绒毛和孢子囊都相关，并且可以通过TEM上存在毛囊虫来区分。观察到大二孢子，但未观察到小二孢子。可能是由于Hematodinium sp。发展到恐龙孢子阶段的文化。如先前在Hematodinium spp中所述，没有观察到早期生命阶段，包括活动性丝状小phon或。来自其他甲壳动物宿主。所有Hematodinium sp。生命阶段包含自发荧光的，与膜结合的电子致密颗粒，在体外培养过程中，它们似乎会脱粒或从细胞中排出。

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