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首页> 外文期刊>Parasitology >N-terminal fusion of a toll-like receptor 2-ligand to a Neospora caninum chimeric antigen efficiently modifies the properties of the specific immune response
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N-terminal fusion of a toll-like receptor 2-ligand to a Neospora caninum chimeric antigen efficiently modifies the properties of the specific immune response

机译：Toll样受体2配体与新孢子虫嵌合抗原的N末端融合可有效修饰特异性免疫应答的特性

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Immunoprophylactic products against neosporosis during pregnancy should induce an appropriately balanced immune response. In this respect, OprI, a bacterial lipoprotein targeting toll like receptor (TLR)2, provides promising adjuvant properties. We report on the manipulation of the innate and the T-cell immune response through the fusion of OprI with the Neospora caninum chimeric protein Mic3-1-R. In contrast to Mic3-1-R, OprI-Mic3-1-R significantly activated bone-marrow dendritic cells from naive mice. Mice immunized with OprI-Mic3-1-R induced an immune response with mixed T helper (Th)1 and Th2 properties (high levels of both immunoglobulin (Ig)G1 and IgG2a and of interleukin (IL)-10, IL-12(p70) and interferon-gamma responses) whereas Mic3-1-R+saponin induced a clear Th2-biased response (low IgG2a and high IL-4 and IL-10). After mating and challenge with N. caninum, increased expression of interferon-gamma was only found in placentas from OprI-Mic3-1-R immunized dams. However, no protection against vertical transmission and neonatal mortality was observed in either of the two groups. These results indicated that more exhaustive studies must be done to elucidate the immune mechanisms associated with transplacental transmission. Antigen linkage to TLR2-ligands, such as OprI, is a useful tool to investigate this enigma by reorienting the innate and adaptive immune responses against other candidate antigens in future studies.
机译：怀孕期间针对新孢子虫病的免疫预防产品应诱导适当平衡的免疫反应。在这方面，OprI是一种靶向脂蛋白样受体（TLR）2的细菌脂蛋白，可提供有希望的佐剂特性。我们报告通过与新孢子虫嵌合蛋白Mic3-1-R OprI融合的先天和T细胞免疫反应的操纵。与Mic3-1-R相反，OprI-Mic3-1-R显着激活了来自幼稚小鼠的骨髓树突状细胞。用OprI-Mic3-1-R免疫的小鼠诱导的免疫反应具有混合的T辅助（Th）1和Th2特性（免疫球蛋白（Ig）G1和IgG2a以及白介素（IL）-10，IL-12（ p70）和干扰素-γ应答），而Mic3-1-R +皂苷诱导了明显的Th2偏向应答（低IgG2a和高IL-4和IL-10）。与犬新孢子虫交配和攻击后，仅在OprI-Mic3-1-R免疫母鼠的胎盘中发现干扰素-γ的表达增加。然而，两组均未观察到针对垂直传播和新生儿死亡率的保护措施。这些结果表明，必须做更详尽的研究以阐明与经胎盘传播有关的免疫机制。与TLR2-配体的抗原连接（例如OprI）是一种有用的工具，可通过在未来的研究中重新定向针对其他候选抗原的先天性和适应性免疫应答来研究此谜。

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