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首页> 外文期刊>Parasitology >Development of a versatile in vitro method for understanding the migration of Fasciola hepatica newly excysted juveniles
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Development of a versatile in vitro method for understanding the migration of Fasciola hepatica newly excysted juveniles

机译：开发一种通用的体外方法来了解新近灭绝的Fasciola hepatica的迁移

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Fasciola hepatica is a parasitic trematode that causes serious losses to livestock producers, and also zoonotic disease. The limitations of chemotherapy for the control of fasciolosis have led to significant interest in the development of vaccines to protect cattle and sheep from infection. However, relatively few studies have concentrated on the mechanisms of invasion of the gut by newly excysted juvenile liver flukes (NEJ) and the host response triggered by this event. The aim of this work was to develop an in vitro model to study invasion by NEJ, while also reducing the requirement for challenge infections of experimental animals. Fasciola hepatica metacercariae were excysted in vitro and placed into compartments containing rat distal jejunal sheets. Variations in incubation medium, chamber size and incubation temperature were used to identify optimal conditions for NEJ migration across the gut. Histological examination showed increased migration until 120 min post-incubation. The use of RPMI, without gassing at 39 degrees C, as the incubation medium was found to be optimal, with 40.5% of NEJ migrating after 150 min. This study describes a readily-reproducible method for studying the migration of F. hepatica NEJ within the definitive host. It will be useful for identifying potential drug and vaccine targets.
机译：肝片Fasciola hepatica是一种寄生性吸虫，会对牲畜生产者造成严重损失，也导致人畜共患病。化学疗法用于控制筋膜炎的局限性引起了人们对开发保护牛和羊免受感染的疫苗的极大兴趣。然而，相对较少的研究集中在新近被囊化的幼虫肝吸虫（NEJ）和由该事件触发的宿主反应对肠道的侵袭机制上。这项工作的目的是建立一个体外模型来研究NEJ的侵袭，同时还减少了对实验动物的挑战性感染的需求。肝虫Fasciola hepatica metacercariae在体外被囊化，并放入含有大鼠空肠远端片的隔室中。孵育培养基，培养箱大小和孵育温度的变化用于确定NEJ在肠道内迁移的最佳条件。组织学检查显示迁移增加，直到孵育后120分钟。发现在39摄氏度下不放气的情况下，使用RPMI作为孵育培养基是最佳的，NEJ的40.5％在150分钟后迁移。这项研究描述了一种易于重现的方法，用于研究最终宿主中肝炎性肝炎NEJ的迁移。这将有助于确定潜在的药物和疫苗目标。

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