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首页> 外文期刊>Parasitology >Molecular characterization and expression analysis of three novel autophagy-related genes from the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)
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Molecular characterization and expression analysis of three novel autophagy-related genes from the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)

机译：牛tick Rhipicephalus（Boophilus）microplus（Acari：Ixodidae）的三个新的自噬相关基因的分子表征和表达分析

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摘要

The cattle tick Rhipicephalus (Boophilus) microplus is a hematophagous ectoparasite of major importance the livestock industry. It shows a remarkable ability to survive over long periods without feeding. However, the mechanisms used to endure long-term starvation are poorly understood. It is believed that autophagy, a process of intracellular protein degradation, may play a significant role to confront adverse environmental conditions. To advance our understanding of autophagy in R. microplus, in the present study we report the molecular characterization of three autophagy-related (ATG) genes, namely, RmATG3, RmATG4 and RmATG6, as well as their expression profiles in different developmental stages and organs of the parasite. The deduced amino acid sequences derived from the characterized gene sequences were subjected to Basic Local Alignment Search Tool analysis. The testing produced significant alignments with respective ATG proteins from Haemaphysalis longicornis and Ixodes scapularis ticks. Real-time polymerase chain reaction assays revealed that be in A TG4 and RmATG6 transcripts were elevated in egg and ovary tissue, when compared with larva and midgut samples, while RmATG3 expression in midgut was 2-fold higher than in egg, larva and ovary samples.
机译：牛tick Rhipicephalus（Boophilus）microplus是一种嗜血的体外寄生虫，对畜牧业具有重要意义。它显示出非凡的长期生存能力。然而，人们对长期忍受饥饿的机制知之甚少。据信自噬是细胞内蛋白质降解的过程，可能在面对不利的环境条件方面起重要作用。为了进一步了解小球藻的自噬，在本研究中，我们报告了三种自噬相关（ATG）基因的分子表征，即RmATG3，RmATG4和RmATG6，以及它们在不同发育阶段和器官中的表达谱的寄生虫。对源自特征基因序列的推导氨基酸序列进行基本局部比对搜索工具分析。该测试与长血ema和肩x小I的各个ATG蛋白产生了显着比对。实时聚合酶链反应分析表明，与幼虫和中肠样品相比，卵和卵巢组织中的TG4和RmATG6转录本升高，而中肠中RmATG3的表达是卵，幼虫和卵巢样品中的2倍。

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