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首页> 外文期刊>Parasitology >Mapping of the conserved antigenic domains shared between potato apyrase and parasite ATP diphosphohydrolases: potential application in human parasitic diseases.
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Mapping of the conserved antigenic domains shared between potato apyrase and parasite ATP diphosphohydrolases: potential application in human parasitic diseases.

机译：马铃薯腺苷三磷酸双磷酸酶和寄生虫ATP二磷酸水解酶之间共享的保守抗原结构域的定位：在人类寄生虫病中的潜在应用。

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摘要

Evolutionary and closer structural relationships are demonstrated by phylogenetic analysis, peptide prediction and molecular modelling between Solanum tuberosum apyrase, Schistosoma mansoni SmATPase 2 and Leishmania braziliensis NDPase. Specific protein domains are suggested to be potentially involved in the immune response, and also seem to be conserved during host and parasite co-evolution. Significant IgG antibody reactivity was observed in sera from patients with American cutaneous leishmaniasis (ACL) and schistosomiasis using potato apyrase as antigen in ELISA. S. mansoni adult worm or egg, L. braziliensis promastigote (Lb) and Trypanosoma cruzi epimastigote (EPI) have ATP diphosphohydrolases, and antigenic preparations of them were evaluated. In ACL patients, IgG seropositivity was about 43% and 90% for Lb and potato apyrase, respectively, while IgM was lower (40%) or IgG (100%) seropositivity for both soluble egg (SEA) and adult worm (SWAP) antigens was higher than that found for potato apyrase (IgM=10%; IgG=39%). In Chagas disease, IgG seropositivity for EPI and potato apyrase was 97% and 17%, respectively, while the IgM was low (3%) for both antigens. The study of the conserved domains from both parasite proteins and potato apyrase could lead to the development of new drug targets or molecular markers.
机译：通过系统发育分析，肽预测和分子建模来证明马铃薯马铃薯红脓病，曼氏血吸虫SmATPase 2和巴西利什曼原虫NDPase之间的进化和更紧密的结构关系。建议特定的蛋白质结构域可能参与免疫反应，并且在宿主和寄生虫共同进化过程中也似乎是保守的。使用马铃薯腺苷三磷酸酶作为抗原在ELISA中在美国皮肤利什曼病（ACL）和血吸虫病患者的血清中观察到了显着的IgG抗体反应性。曼氏沙门氏菌成虫或卵，巴西乳杆菌promastigote（Lb）和克氏锥虫锥虫（EPI）具有ATP二磷酸水解酶，并对其抗原制剂进行了评估。在ACL患者中，Lb和马铃薯腺苷三磷酸酶的IgG血清阳性率分别约为43％和90％，而可溶性蛋（SEA）和成虫（SWAP）抗原的IgM血清阳性率则分别较低（40％）或IgG（100％）。高于马铃薯腺苷三磷酸酶的发现（IgM = 10％; IgG = 39％）。在恰加斯病中，对EPI和马铃薯腺苷三磷酸酶的IgG血清阳性分别为97％和17％，而两种抗原的IgM都很低（3％）。对来自寄生虫蛋白和马铃薯腺苷三磷酸酶的保守结构域的研究可能导致开发新的药物靶标或分子标记。

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