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首页> 外文期刊>Parasitology >Post-surgical follow-up (by ELISA and immunoblotting) of cured versus non-cured cystic echinococcosis in young patients.
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Post-surgical follow-up (by ELISA and immunoblotting) of cured versus non-cured cystic echinococcosis in young patients.

机译：年轻患者中治愈与未治愈的囊性棘球病的手术后随访（通过ELISA和免疫印迹）。

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The study was designed to determine comparatively the prognostic value of immunoblotting and ELISA in the serological follow-up of young cystic echinococcosis (CE) patients exhibiting either a cured or a progredient (non-cured) course of disease after treatment. A total of 54 patients (mean age 9 years, range from 3 to 15 years) with surgically, radiologically and/or histologically proven CE were studied for a period up to 60 months after surgery. Additionally, some of the patients underwent chemotherapy. Based on the clinical course and outcome, as well as on imaging findings, patients were clustered into 2 groups of either cured (CCE), or non-cured (NCCE) CE patients. ELISA showed a high rate of seropositivity 4 to 5 years post-surgery for both CCE (57.1%) and NCCE (100%) patients, the difference found between the two groups was statistically not significant. Immunoblotting based upon recognition of AgB subcomponents (8 and 16 kDa bands) showed a decrease of respective antibody reactivities after 4 years post-surgery. Only sera from 14.3% of CCE patients recognized the subcomponents of AgB after 4 years, while none (0%) of these sera was still reactive at 5 years post-surgery. At variance, immunoblotting remained positive for AgB subcomponents in 100% of the NCCE cases as tested between 4 and 5 years after surgical treatment. Immunoblotting therefore proved to be a useful approach for monitoring post-surgical follow-ups of human CCE and NCCE in young patients when based upon the recognition of AgB subcomponents.
机译：该研究旨在确定免疫印迹和ELISA在年轻的囊性包虫病（CE）患者在治疗后表现出治愈或进展性（非治愈）过程的血清学随访中的预后价值。对总共54例经手术，放射学和/或组织学证实为CE的患者（平均年龄9岁，范围从3至15岁）进行了研究，研究期长达60个月。另外，一些患者接受了化疗。根据临床过程和结果以及影像学发现，将患者分为两组，即治愈的（CCE）或未治愈的（NCCE）CE患者。 ELISA显示CCE（57.1％）和NCCE（100％）病人术后4至5年的血清阳性率很高，两组之间的差异在统计学上不显着。免疫印迹基于对AgB子成分（8和16 kDa条带）的识别，显示术后4年后各自的抗体反应性降低。 4年后，只有14.3％的CCE患者血清能识别到AgB的亚成分，而这些血清在术后5年仍无反应（0％）。经过方差分析，在手术治疗后4至5年间，在100％的NCCE病例中，AgB子成分的免疫印迹仍为阳性。因此，基于对AgB亚组分的识别，免疫印迹被证明是监测年轻患者中人CCE和NCCE的术后随访的有用方法。

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