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首页> 外文期刊>Parasitology >Effect of acaricides on the activity of glutathione transferases from the parasitic mite Sarcoptes scabiei
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Effect of acaricides on the activity of glutathione transferases from the parasitic mite Sarcoptes scabiei

机译：杀螨剂对sc螨Sarcoptes scabiei谷胱甘肽转移酶活性的影响

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摘要

Glutathione transferases (GSTs) are a family of multifunctional enzymes with fundamental roles in cellular detoxication. Here we report the molecular characterization of 3 recombinant GSTs belonging to the mu- and delta-class from the parasitic mite Sarcoptes scabiei. Kinetic constants were determined, and the effect of acaricides, including organothio-phosphates, pyrethroid esters, a formamidine, a macrocyclic lactone, an organochlorine as well as a bridged diphenyl acaricide, on the activity of the GSTs were tested using l-chloro-2,4-dinitrobenzene (CDNB) as model substrate. Our results showed that enzymes from the same class and with high amino acid sequence identity have significantly different kinetic properties. For instance, one mu-class GST lost more than 50% of its activity in the presence of one of the organothiophosphates while the activity of the second mu-class GST was only slightly reduced under identical conditions. Tertiary structure modulations indicated that structural differences were the crucial factor for the different kinetic patterns observed. Genome analysis showed that the two mu-class GSTs are organized in tandem in the S. scabiei genome. Taken together these results show that GSTs might be involved in the metabolism of acaricides in S. scabiei.
机译：谷胱甘肽转移酶（GST）是一类多功能酶，在细胞解毒中起着基本作用。在这里，我们报告从寄生螨Sarcoptes scabiei属于mu和delta类的3个重组GST的分子表征。确定了动力学常数，并使用1-氯-2检验了包括有机硫代磷酸盐，拟除虫菊酯，甲am，大环内酯，有机氯以及桥联二苯杀螨剂在内的杀螨剂对GST活性的影响。 ，4-二硝基苯（CDNB）作为模型底物。我们的结果表明，来自同一类别且具有高度氨基酸序列同一性的酶具有显着不同的动力学特性。例如，一个mu-class GST在一种有机硫代磷酸酯的存在下损失了超过50％的活性，而第二个mu-class GST在相同条件下的活性仅略有降低。三级结构调节表明，结构差异是观察到的不同动力学模式的关键因素。基因组分析表明，两个mu-class GSTs在S. scabiei基因组中串联排列。综上所述，这些结果表明，GSTs可能与S. scabiei中的杀螨剂代谢有关。

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