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首页> 外文期刊>Parasitology >Molecular evidence of prevalent dual piroplasma infections in North American raccoons (Procyon lotor).
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Molecular evidence of prevalent dual piroplasma infections in North American raccoons (Procyon lotor).

机译：北美浣熊（Procyon lotor）中普遍存在的双重螺旋体感染的分子证据。

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摘要

Based on 18S rRNA sequence analyses 2 distinct genotypes of piroplasms have been described in raccoons. One genotype resides in the Babesia sensu stricto clade and the other in the Babesia microti-like clade. Since these organisms appear morphologically indistinguishable, it is unclear which strain is responsible for the majority of the infections in raccoons. In order to overcome these limitations we performed a molecular survey of raccoons using polymerase chain reaction assays specific for each genotype. We tested blood samples from 41 wild raccoons trapped in eastern North Carolina using PCR assays and found that 95% (39/41) had detectable piroplasm DNA. Ninety percent (37/41) of the samples contained Babesia sensu stricto DNA and 83% (34/41) samples contained Babesia microti-like DNA. DNA from both genotypes was present in 76% (31/41) samples suggesting a very high rate of co-infections. The presence of dual piroplasma infections in carnivores appears to be an uncommon finding. This study highlights the need for molecular assays for the accurate identification of piroplasma. Further studies are indicated to investigate the ability of these parasites to infect domestic animals as well as their zoonotic potential.
机译：基于18S rRNA序列分析，浣熊中已经描述了2种不同的子质基因型。一种基因型存在于严格的巴贝斯氏森（Babesia sensu）进化枝中，另一种基因型则存在于巴贝斯虫（Babesia）的小肠样小枝中。由于这些生物在形态上似乎无法区分，因此尚不清楚哪种菌株负责浣熊的大多数感染。为了克服这些限制，我们使用了针对每种基因型的聚合酶链反应测定法对浣熊进行了分子调查。我们使用PCR方法检测了北卡罗莱纳州东部捕获的41只野生浣熊的血样，发现95％（39/41）的可检测到的质体DNA。百分之九十（37/41）的样本中包含严格的巴比斯虫DNA，而百分之八十三（34/41）的样本中包含巴比斯虫的微粒状DNA。来自这两种基因型的DNA存在于76％（31/41）的样品中，表明合并感染率很高。食肉动物中双螺旋体感染的存在似乎并不常见。这项研究强调了分子检测以准确鉴定螺胞质的需求。指示需要进一步研究以调查这些寄生虫感染家畜的能力及其人畜共患病潜力。

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