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首页> 外文期刊>Parasitology >Physiological and morphological effects of genistein against the liver fluke, Fasciola hepatica.
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Physiological and morphological effects of genistein against the liver fluke, Fasciola hepatica.

机译：金雀异黄素对肝吸虫Fasciola hepatica的生理和形态学影响。

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A study has been carried out to determine the activity of genistein against adult liver fluke, Fasciola hepatica. Flukes were incubated in vitro in genistein at a concentration of 0.27 mg/ml (=1 mm). They ceased to move after 3 h, at which point the experiment was terminated and the specimens prepared for examination by scanning and transmission electron microscopy. Surface changes to the flukes comprised swelling and blebbing, especially in the posterior region of the flukes, and there was particular disruption to the spines, accompanied by some spine loss. Fine structural changes to the tegumental syncytium indicated an accelerated release of secretory bodies at the surface, but a reduction in their production within the cell bodies. Autophagic activity was evident in the tegumental cells, a phenomenon that was also observed in the gastrodermal cells. Disruption to the testis and vitelline follicles was severe, with an apparent block in the normal developmental sequence of the spermatogenic and vitelline cells, respectively. Shell protein production by the vitelline cells was also disrupted. In separate experiments, somatic muscle strips were exposed to concentrations of genistein ranging from 1 microm to 1 mm. There were statistically significant increases in the frequency and/or amplitude of muscle contractions at concentrations of 10 microm, 100 microm and 1 mm. The results suggest that genistein is capable of causing severe morphological and neuromuscular disruption to adult flukes in vitro over a short time-span.
机译：已经进行了一项研究以确定染料木黄酮对成年肝吸虫Fasciola hepatica的活性。将鳞片在染料木黄酮中以0.27 mg / ml（= 1 mm）的浓度进行体外培养。 3小时后它们不再移动，这时实验终止，并准备通过扫描和透射电子显微镜检查样品。吸虫的表面变化包括肿胀和起泡，尤其是在吸虫的后部区域，尤其是刺棘受到破坏，并伴有一些脊柱丢失。皮膜合胞体的精细结构变化表明分泌体在表面加速释放，但在细胞体内的分泌减少。自噬活性在外皮细胞中很明显，这种现象在胃皮细胞中也观察到。睾丸和卵黄卵泡的破坏很严重，分别在生精和卵黄细胞的正常发育过程中出现明显的阻滞。卵黄细胞产生的壳蛋白也被破坏。在单独的实验中，将体细胞条暴露于染料木黄酮的浓度范围为1微米至1毫米。在浓度为10微米，100微米和1毫米时，肌肉收缩的频率和/或幅度在统计学上显着增加。结果表明，金雀异黄素能够在短时间范围内对成年吸虫造成严重的形态和神经肌肉破坏。

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