...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Parasitology >Stage-specific expression of Nc SAG4 as a marker of chronic Neospora caninum infection in a mouse model.
【24h】

Stage-specific expression of Nc SAG4 as a marker of chronic Neospora caninum infection in a mouse model.

机译：Nc SAG4的阶段特异性表达在小鼠模型中作为慢性新孢子虫感染的标志物。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Neospora caninum infection persists throughout the life of its intermediate host due to the conversion of tachyzoites to slowly dividing bradyzoites that encyst in the brain. This event results in persistent N. caninum infection in bovine herds and partially explains the poor efficacy of many chemotherapeutic agents and vaccine formulations. Thus, there is a need for greater understanding of the tachyzoite-to-bradyzoite conversion mechanisms. Here we studied for the first time the transcription kinetics of the N. caninum bradyzoite-specific gene NcSAG4 in brain samples from chronically infected mice by means of real-time RT-PCR. NcSAG4-messenger RNA (mRNA) levels increased significantly during the chronic phase but followed 2 different expression patterns depending on the isolate used for murine inoculation. NcSAG4-mRNA levels in brains from Nc-1-inoculated mice peaked during late chronic infection (on day 64 post-infection, p.i.), whereas those from Nc-Liv-inoculated mice peaked earlier during the chronic infection (on day 32 p.i.). This difference could be a reflection of the different abilities of these isolates to replicate and form cysts in parasitized brains. These results are consistent with our observations of anti-rNcSAG4 antibody production; low levels were present at seroconversion and slowly increased during the chronic phase. In contrast, NcSAG1 transcription levels, which mark the tachyzoite stage, were maintained without variation in both groups of mice. This suggests the presence of a significant amount of tachyzoites or intermediate zoites expressing NcSAG1 in the brain, even during the late chronic infection.
机译：犬新孢子虫感染在其中间宿主的整个生命中持续存在，这是由于速殖子转变为缓慢分裂的缓殖子，它们包裹在大脑中。此事件导致牛群中持续发生犬新孢子虫感染，部分解释了许多化学治疗剂和疫苗制剂的不良功效。因此，需要对速殖子到缓殖子的转化机理有更多的了解。在这里，我们首次通过实时RT-PCR研究了慢性感染小鼠大脑样本中犬新孢子虫缓殖子特异基因NcSAG4的转录动力学。 NcSAG4-信使RNA（mRNA）水平在慢性期明显增加，但遵循两种不同的表达方式，具体取决于用于鼠类接种的分离株。 Nc-1接种小鼠的大脑中NcSAG4-mRNA水平在慢性感染后期（感染后第64天，pi）达到峰值，而Nc-Liv接种小鼠的大脑中的NcSAG4-mRNA水平在慢性感染期间（感染后32天）达到峰值。 。这种差异可能反映了这些分离株在被寄生的大脑中复制和形成囊肿的能力不同。这些结果与我们对抗rNcSAG4抗体产生的观察结果一致。血清转化时水平低，在慢性期缓慢增加。相反，在两组小鼠中，维持标记速殖子阶段的NcSAG1转录水平不变。这表明即使在晚期慢性感染期间，大脑中仍存在大量表达NcSAG1的速殖子或中间动物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号