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首页> 外文期刊>Parasitology >Transcriptome analysis of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE).
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Transcriptome analysis of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE).

机译：使用基因表达系列分析（SAGE）的曼氏血吸虫幼虫发育的转录组分析。

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SUMMARY: Infection of the snail, Biomphalaria glabrata, by the free-swimming miracidial stage of the human blood fluke, Schistosoma mansoni, and its subsequent development to the parasitic sporocyst stage is critical to establishment of viable infections and continued human transmission. We performed a genome-wide expression analysis of the S. mansoni miracidia and developing sporocyst using Long Serial Analysis of Gene Expression (LongSAGE). Five cDNA libraries were constructed from miracidia and in vitro cultured 6- and 20-day-old sporocysts maintained in sporocyst medium (SM) or in SM conditioned by previous cultivation with cells of the B. glabrata embryonic (Bge) cell line. We generated 21 440 SAGE tags and mapped 13 381 to the S. mansoni gene predictions (v4.0e) either by estimating theoretical 3' UTR lengths or using existing 3' EST sequence data. Overall, 432 transcripts were found to be differentially expressed amongst all 5 libraries. In total, 172 tags were differentially expressed between miracidia and 6-day conditioned sporocysts and 152 were differentially expressed between miracidia and 6-day unconditioned sporocysts. In addition, 53 and 45 tags, respectively, were differentially expressed in 6-day and 20-day cultured sporocysts, due to the effects of exposure to Bge cell-conditioned medium.
机译：摘要：人类血吸虫曼氏血吸虫的自由游动的水acid阶段感染了蜗牛glabrata glabrata，随后其发展为寄生的孢子囊期对于建立可行的感染和持续的人类传播至关重要。我们使用长序列基因表达分析（LongSAGE）对曼氏葡萄球菌嗜酸菌和发育的孢子囊进行了全基因组表达分析。从羽球菌中构建了五个cDNA文库，并体外培养了6天和20天大的孢子囊，这些孢子囊保存在孢子囊培养基（SM）或通过预先用光滑芽孢杆菌胚胎（Bge）细胞系培养的条件下的SM中。我们通过估计理论3'UTR长度或使用现有的3'EST序列数据，生成了21 440个SAGE标签，并将13 381映射到曼氏葡萄球菌基因预测（v4.0e）。总体而言，发现在所有5个文库中有432个转录物差异表达。总共172个标签在miracidia和6天条件性孢子囊之间差异表达，并且152个标签在miracidia和6天非条件性孢子囊之间差异表达。此外，由于暴露于Bge细胞条件培养基的影响，分别在6天和20天培养的孢子囊中差异表达了53和45个标签。

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