An alternative method for DNA extraction and PCR identification of Entamoeba histolytica and E. dispar in fecal samples.

Vianna EN; Costa JO; Santos CK

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An alternative method for DNA extraction and PCR identification of Entamoeba histolytica and E. dispar in fecal samples.

机译：粪便样品中溶组织变形杆菌和分离的大肠杆菌的DNA提取和PCR鉴定的另一种方法。

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Since it is known that Entamoeba dispar is non-pathogenic and morphologically similar to E. histolytica, there are many targets used in PCR for differentiating these species. However, obtaining high quality DNA from fecal samples is fundamental for PCR. Most methods are laborious or use kits that make diagnosis expensive. In the present work, a new simple, fast and cheap technique of DNA extraction from fecal samples was combined with a PCR for an episomal target in order to identify E. histolytica and E. dispar in feces.

机译：由于已知Entamoeba dispar是非病原性的，并且在形态学上类似于组织溶大肠杆菌，因此在PCR中有许多用于区分这些物种的靶标。但是，从粪便样品中获得高质量的DNA是PCR的基础。大多数方法费力或使用试剂盒会使诊断昂贵。在目前的工作中，从粪便样品中提取DNA的一种简单，快速和廉价的新技术与一种针对游离目标的PCR结合在一起，以鉴定粪便中的组织溶大肠杆菌和分离的大肠杆菌。

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《Parasitology》 |2009年第7期|共5页
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Vianna EN; Costa JO; Santos CK;
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正文语种 eng
中图分类医学寄生虫学;寄生虫病;
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1. An alternative method for DNA extraction and PCR identification of Entamoeba histolytica and E. dispar in fecal samples. [J] . Vianna EN, Costa JO, Santos CK Parasitology . 2009,第7期

机译：粪便样品中溶组织变形杆菌和分离的大肠杆菌的DNA提取和PCR鉴定的另一种方法。
2. A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica , E. moshkovskii and E. dispar DNA in stool samples [J] . Krishna Khairnar, Subhash C Parija BMC Microbiology . 2007,第1期

机译：一种新颖的巢式多重聚合酶链反应（PCR）分析法，用于粪便样品中的溶组织性变形杆菌，moshkovskii和dispar DNA的差异检测
3. A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica , E. moshkovskii and E. dispar DNA in stool samples [J] . Krishna Khairnar, Subhash C Parija BMC Microbiology . 2007,第1期

机译：一种新颖的巢式多重聚合酶链反应（PCR）分析法，用于粪便样品中的溶组织性变形杆菌，moshkovskii和dispar DNA的差异检测
4. Prevalence of Amoebiasis by Entamoeba Histolytica/E. Dispar in Three Regions of Peru [C] . M. Beltran, M. Garaycochea, N. Bellido, International Congress of Parasitology . 2006

机译：entamoeba组织olytica / e的amoebiasis患病率。在秘鲁的三个地区贬低
5. Identification of unknown PCR products generated during STR analysis of DNA from bone samples. [D] . Koehn, Alyssa. 2013

机译：鉴定在对骨骼样品中的DNA进行STR分析时生成的未知PCR产物。
6. A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica E. moshkovskii and E. dispar DNA in stool samples [O] . Krishna Khairnar, Subhash C Parija 2007

机译：一种新型的巢式多重聚合酶链反应（PCR）分析法用于粪便样品中的溶组织变形杆菌moshkovskii和dispar DNA的差异检测
7. A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica, E. moshkovskii and E. dispar DNA in stool samples [O] . Khairnar, Krishna, Parija, Subhash C 2007

机译：一种新型的巢式多重聚合酶链反应（PCR）分析法，用于粪便样品中的溶组织变形杆菌，moshkovskii和dispar DNA的差异检测

An alternative method for DNA extraction and PCR identification of Entamoeba histolytica and E. dispar in fecal samples.

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