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首页> 外文期刊>Parasitology >Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species.
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Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species.

机译：分子证据表明，来自实验室小鼠和木材小鼠的多头螺旋藻是分开的物种。

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摘要

The gastro-intestinal (GI) nematode Heligmosomoides polygyrus is an important experimental model in laboratory mice and a well-studied parasite of wood mice in the field. Despite an extensive literature, the taxonomy of this parasite in different hosts is confused, and it is unclear whether laboratory and field systems represent the same or different Operational Taxonomic Units (OTUs). Molecular analyses reveal high sequence divergence between H. p. bakeri (laboratory) and H. p. polygyrus (field); 3% difference in the ribosomal DNA Internal Transcribed Spacers (ITS) and 8.6% variation in the more rapidly evolving mitochondrial cytochrome c oxidase I (COI) gene. The COI sequence of U.K. H. p. polygyrus is more similar to H. glareoli from voles than to H. p. bakeri, while a single isolate of H. p. polygyrus from Guernsey confirms the extent of genetic variation between H. p. polygyrus populations. Analysis of molecular variance demonstrated that mtCOI sequence variation is associated primarily with groups with distinct ITS2 sequences, and with host identity, but is not partitioned significantly with a single combined taxon H. polygyrus incorporating European and North American isolates. We conclude therefore that the laboratory OTU should be raised to the level of a distinct species, as H. bakeri from the laboratory mouse Mus musculus, and we reject the hypothesis that H. bakeri has diverged from H. polygyrus in the recent past following introduction into America. However, we are unable to reject the hypothesis that H. polygyrus and H. bakeri are sister taxa, and it may be that H. polygyrus is polyphyletic or paraphyletic.
机译：胃肠线虫（GI）线虫多回是在实验室小鼠中的重要实验模型，也是野外对木材小鼠进行充分研究的寄生虫。尽管有大量文献报道，但该寄生虫在不同宿主中的分类法还是很混乱的，目前尚不清楚实验室和现场系统是代表相同还是不同的操作分类单位（OTU）。分子分析显示H. p。之间的高度序列差异。面包房（实验室）和H. p。多头回（田）;核糖体DNA内部转录间隔区（ITS）的差异为3％，而线粒体细胞色素C氧化酶I（COI）基因的快速进化为8.6％。英国H.p.的COI序列从田鼠看，多头回比得上H. glareoli比H. p。更像。面包，而H. p。根西岛的多回合证实了H. p。之间的遗传变异程度。多回合种群。分子变异分析表明，mtCOI序列变异主要与具有不同ITS2序列的基团和宿主身份相关，但对于合并了欧洲和北美分离株的单一分类群H. polygyrus却没有明显的划分。因此，我们得出结论，应将实验室OTU升高至不同物种的水平，例如来自实验室小鼠Mus mus mus的面包酵母H. Bakeri，并且我们拒绝以下假设：在引入后的近期内，面包酵母H. polygyrus偏离了进入美国。但是，我们无法拒绝假单胞菌和面包师傅是姊妹类群的假说，可能是多回菌是多系的或共生的。

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