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首页> 外文期刊>Parasitology >Schistosoma japonicum triose-phosphate isomerase plasmid DNA vaccine protects pigs against challenge infection.
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Schistosoma japonicum triose-phosphate isomerase plasmid DNA vaccine protects pigs against challenge infection.

机译：日本血吸虫三磷酸磷酸酯酶异构酶质粒DNA疫苗可保护猪免于感染。

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摘要

The protective efficacy of a Schistosoma japonicum, Chinese strain, triose-phosphate isomerase (TPI) plasmid DNA vaccine was examined in naive pigs. Pigs were vaccinated with the TPI DNA-plasmid alone, or in conjunction with IL-12 as pcDNA3.1-P35, pcDNA3.1-P40 plasmids via intramuscular injection. Control pigs were immunized with equivalent amounts of pcDNA3.1. Pigs were immunized 3 times at 21-day intervals and challenged 30 days after the final boost. Forty-five days post-challenge, pigs were sacrificed and perfused to compare adult worm burdens, female worm burdens, liver egg burdens and granuloma size. We found that pigs vaccinated with SjCTPI DNA alone had adult worm burdens reduced by 48.3% and that a further decrease in adult worm burdens was not seen in the group vaccinated with SjCTPI DNA in conjunction with IL-12 (46.2% reduction). The SjCTPI DNA vaccines had a more pronounced effect on reducing female worm burdens i.e. 53.6% SjCTPI alone and 59.6% for SjCTPI+IL-12. Vaccination with SjCTPI-DNA reduced liver eggs by 49.4% and this response was significantly enhanced by the addition of IL-12 (65.8% reduction in liver eggs). In addition to the dramatic protective effects seen in vaccinated pigs, we also noted that granuloma size was reduced by 42% in both groups. Thus, vaccination of pigs and other large animals in China with SjCTPI DNA vaccine will likely reduce transmission by reducing adult worm burdens and worm egg output and simultaneously reduce hepatic egg-associated pathology.
机译：在幼稚猪中检查了日本血吸虫中国菌株三糖磷酸异构酶（TPI）质粒DNA疫苗的保护作用。通过肌肉注射将猪的TPI DNA质粒单独或与IL-12一起接种，分别作为pcDNA3.1-P35，pcDNA3.1-P40质粒。用等量的pcDNA3.1免疫对照猪。猪以21天的间隔免疫3次，并在最终加强免疫后30天进行攻击。攻击后四十五天，将猪处死并灌注以比较成年蠕虫负担，雌性蠕虫负担，肝卵负担和肉芽肿大小。我们发现，仅接种了SjCTPI DNA的猪的成虫蠕虫负担降低了48.3％，而接种了SjCTPI DNA和IL-12的疫苗组未发现成虫蠕虫负担进一步降低（降低了46.2％）。 SjCTPI DNA疫苗在减少雌虫蠕虫负担方面具有更明显的效果，即单独使用SjCTPI的53.6％和使用SjCTPI + IL-12的59.6％。用SjCTPI-DNA进行疫苗接种可使肝卵减少49.4％，并且通过添加IL-12（肝卵减少65.8％）显着增强了这种反应。除了在接种疫苗的猪中看到了显着的保护作用外，我们还注意到两组肉芽肿的大小均减少了42％。因此，用SjCTPI DNA疫苗对中国的猪和其他大型动物进行疫苗接种可能会通过减少成年蠕虫负担和蠕虫卵产量来减少传播，同时减少与肝卵相关的病理。

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